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Bacterial and archaeal diversities in Yunnan and Tibetan hot springs, China

Authors

  • Zhao-Qi Song,

    1. Key Laboratory of Microbial Diversity in Southwest China, Ministry of Education, Laboratory for Conservation and Utilization of Bio-resources, Yunnan Institute of Microbiology, Yunnan University, Kunming, China
    2. Engineering Technology Research Center of Biomass Degradation and Gasification, University of Henan Province, Shangqiu Normal University, Shangqiu, China
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    • These authors contributed equally to this work.
  • Feng-Ping Wang,

    1. Key Laboratory of MOE for Microbial metabolism and School of Life Sciences and Biotechnology, State Key Laboratory of Ocean Engineering, Shanghai Jiao Tong University, Shanghai, China
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    • These authors contributed equally to this work.
  • Xiao-Yang Zhi,

    1. Key Laboratory of Microbial Diversity in Southwest China, Ministry of Education, Laboratory for Conservation and Utilization of Bio-resources, Yunnan Institute of Microbiology, Yunnan University, Kunming, China
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  • Jin-Quan Chen,

    1. Key Laboratory of Microbial Diversity in Southwest China, Ministry of Education, Laboratory for Conservation and Utilization of Bio-resources, Yunnan Institute of Microbiology, Yunnan University, Kunming, China
    2. Key Laboratory of MOE for Microbial metabolism and School of Life Sciences and Biotechnology, State Key Laboratory of Ocean Engineering, Shanghai Jiao Tong University, Shanghai, China
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  • En-Min Zhou,

    1. Key Laboratory of Microbial Diversity in Southwest China, Ministry of Education, Laboratory for Conservation and Utilization of Bio-resources, Yunnan Institute of Microbiology, Yunnan University, Kunming, China
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  • Feng Liang,

    1. Engineering Technology Research Center of Biomass Degradation and Gasification, University of Henan Province, Shangqiu Normal University, Shangqiu, China
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  • Xiang Xiao,

    1. Key Laboratory of MOE for Microbial metabolism and School of Life Sciences and Biotechnology, State Key Laboratory of Ocean Engineering, Shanghai Jiao Tong University, Shanghai, China
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  • Shu-Kun Tang,

    1. Key Laboratory of Microbial Diversity in Southwest China, Ministry of Education, Laboratory for Conservation and Utilization of Bio-resources, Yunnan Institute of Microbiology, Yunnan University, Kunming, China
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  • Hong-Chen Jiang,

    Corresponding author
    1. State Key Laboratory of Biogeology and Environmental Geology, China University of Geosciences, Wuhan, China
    • Key Laboratory of Microbial Diversity in Southwest China, Ministry of Education, Laboratory for Conservation and Utilization of Bio-resources, Yunnan Institute of Microbiology, Yunnan University, Kunming, China
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  • Chuanlun L. Zhang,

    1. Department of Marine Sciences, University of Georgia, Athens, GA, USA
    2. State Key Laboratory of Marine Geology, Tongji University, Shanghai, China
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  • Hailiang Dong,

    1. State Key Laboratory of Biogeology and Environmental Geology, China University of Geosciences, Wuhan, China
    2. Department of Geology and Environmental Earth Science, Miami University, Oxford, OH, USA
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  • Wen-Jun Li

    Corresponding author
    1. Key Laboratory of Biogeography and Bioresource in Arid Land, Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences, Ürűmqi, China
    • Key Laboratory of Microbial Diversity in Southwest China, Ministry of Education, Laboratory for Conservation and Utilization of Bio-resources, Yunnan Institute of Microbiology, Yunnan University, Kunming, China
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For correspondence. E-mail wjli@ynu.edu.cn; Tel. (+86) 871 5033335; Fax (+86) 871 5033335; E-mail hongchen.jiang@gmail.com; Tel. (+86) 27 67883061; Fax (+86) 27 67883451.

Summary

Thousands of hot springs are located in the north-eastern part of the Yunnan–Tibet geothermal zone, which is one of the most active geothermal areas in the world. However, a comprehensive and detailed understanding of microbial diversity in these hot springs is still lacking. In this study, bacterial and archaeal diversities were investigated in 16 hot springs (pH 3.2–8.6; temperature 47–96°C) in Yunnan Province and Tibet, China by using a barcoded 16S rRNA gene-pyrosequencing approach. Aquificae, Proteobacteria, Firmicutes, Deinococcus-Thermus and Bacteroidetes comprised the large portion of the bacterial communities in acidic hot springs. Non-acidic hot springs harboured more and variable bacterial phyla than acidic springs. Desulfurococcales and unclassified Crenarchaeota were the dominated groups in archaeal populations from most of the non-acidic hot springs; whereas, the archaeal community structure in acidic hot springs was simpler and characterized by Sulfolobales and Thermoplasmata. The phylogenetic analyses showed that Aquificae and Crenarchaeota were predominant in the investigated springs and possessed many phylogenetic lineages that have never been detected in other hot springs in the world. Thus findings from this study significantly improve our understanding of microbial diversity in terrestrial hot springs.

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