These co-authors have equally contributed to this study.
Analysis of the plant growth-promoting properties encoded by the genome of the rhizobacterium Pseudomonas putida BIRD-1
Article first published online: 4 DEC 2012
© 2012 Society for Applied Microbiology and Blackwell Publishing Ltd
Special Issue: Plant–Microbe Interactions
Volume 15, Issue 3, pages 780–794, March 2013
How to Cite
Roca, A., Pizarro-Tobías, P., Udaondo, Z., Fernández, M., Matilla, M. A., Molina-Henares, M. A., Molina, L., Segura, A., Duque, E. and Ramos, J.-L. (2013), Analysis of the plant growth-promoting properties encoded by the genome of the rhizobacterium Pseudomonas putida BIRD-1. Environmental Microbiology, 15: 780–794. doi: 10.1111/1462-2920.12037
- Issue published online: 4 MAR 2013
- Article first published online: 4 DEC 2012
- Accepted manuscript online: 5 NOV 2012 06:50AM EST
- Manuscript Accepted: 23 OCT 2012
- Manuscript Revised: 17 OCT 2012
- Manuscript Received: 31 AUG 2012
- Fondo Social Europeo and FEDER Funds
- Programa Campus of Junta de Andalucía. Grant Number: SV40
- Agencia IDEA
- Bio-Iliberis R&D
- Ministry of Science and Innovation. Grant Number: BIO2010-17227
Fig. S1. Growth of Pseudomonas putida BIRD-1 with different sources of inorganic phosphorous. We used A medium with the indicated C source and insoluble tricalcium phosphate (500 mg l−1) as a source of phosphate. Viable cells at the beginning of the assay were 2 to 3 × 107 cfu ml−1. After 24 h incubation at 30°C the cfu ml−1 was determined after spreading serial dilutions on LB medium. The control is M9 medium with 50 mM soluble phosphate.
Fig. S2. Pikovskaya test with BIRD-1 and mutants in the Entner–Doudoroff pathway. Plates were prepared as described in Experimental procedures. We inoculated cells using a toothpick in a single point on the agar surface. Plates were incubated for 48 h at 30°C and then the solubilization halo was visualized. (A) Wild-type; (B) zwf mutant and (C) eda mutant.
Fig. S3. Growth of P. putida with phytate. Assays were performed with A medium containing 1 g l−1 phytate. At the indicated times cfu ml−1 were determined. As a control, cells growing on M9 minimal medium were used. ○, growth in M9 medium; ▴, growth with phytate.
Fig. S4. Production of IAA by wild-type BIRD-1 and different isogenic mutants. IAA concentration was determined as described in Experimental procedures. The solid back bar represents the wild-type strains and the other bars the indicated mutants.
Fig. S5. Corn root development with mutants in the IAA biosynthetic pathways. The picture shows controls without bacteria (A), with wild-type BIRD-1 (B) and KT2440 (C) strains, and three isogenic mutants of BIRD-1 with knockouts in PPU_BIRD-1_0418, 1202 and 3125 genes (D, E and F respectively).
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Fig. S6. Corn root development in a control seed without bacteria (A), and inoculated seeds with BIRD-1 (B) and a mutant deficient in the acc deaminase gene (C). Conditions were as described for Fig. 2 except that a knockout (PPU_BIRD-1_3642) acc mutant was used.
Table S1. Genes involved in adhesion to biotic and abiotic surfaces in Pseudomonas putida strain BIRD-1 deduced from the identification of reciprocal genes in P. putida KT2440.
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