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emi12037-sup-0001-fs1.TIF2755K

Fig. S1. Growth of Pseudomonas putida BIRD-1 with different sources of inorganic phosphorous. We used A medium with the indicated C source and insoluble tricalcium phosphate (500 mg l−1) as a source of phosphate. Viable cells at the beginning of the assay were 2 to 3 × 107 cfu ml−1. After 24 h incubation at 30°C the cfu ml−1 was determined after spreading serial dilutions on LB medium. The control is M9 medium with 50 mM soluble phosphate.

emi12037-sup-0002-fs2.tif1157K

Fig. S2. Pikovskaya test with BIRD-1 and mutants in the Entner–Doudoroff pathway. Plates were prepared as described in Experimental procedures. We inoculated cells using a toothpick in a single point on the agar surface. Plates were incubated for 48 h at 30°C and then the solubilization halo was visualized. (A) Wild-type; (B) zwf mutant and (C) eda mutant.

emi12037-sup-0003-fs3.TIF2659K

Fig. S3. Growth of P. putida with phytate. Assays were performed with A medium containing 1 g l−1 phytate. At the indicated times cfu ml−1 were determined. As a control, cells growing on M9 minimal medium were used. ○, growth in M9 medium; ▴, growth with phytate.

emi12037-sup-0004-fs4.TIF2890K

Fig. S4. Production of IAA by wild-type BIRD-1 and different isogenic mutants. IAA concentration was determined as described in Experimental procedures. The solid back bar represents the wild-type strains and the other bars the indicated mutants.

emi12037-sup-0005-fs5.tif1359K

Fig. S5. Corn root development with mutants in the IAA biosynthetic pathways. The picture shows controls without bacteria (A), with wild-type BIRD-1 (B) and KT2440 (C) strains, and three isogenic mutants of BIRD-1 with knockouts in PPU_BIRD-1_0418, 1202 and 3125 genes (D, E and F respectively).

emi12037-sup-0006- fs6.tif736K

Fig. S6. Corn root development in a control seed without bacteria (A), and inoculated seeds with BIRD-1 (B) and a mutant deficient in the acc deaminase gene (C). Conditions were as described for Fig. 2 except that a knockout (PPU_BIRD-1_3642) acc mutant was used.

emi12037-sup-0007-ts1.docx17K

Table S1. Genes involved in adhesion to biotic and abiotic surfaces in Pseudomonas putida strain BIRD-1 deduced from the identification of reciprocal genes in P. putida KT2440.

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