Spatial mapping of extracellular oxidant production by a white rot basidiomycete on wood reveals details of ligninolytic mechanism
Version of Record online: 4 DEC 2012
Published 2012. This article is a U.S. Government work and is in the public domain in the USA
Special Issue: Plant–Microbe Interactions
Volume 15, Issue 3, pages 956–966, March 2013
How to Cite
Hunt, C. G., Houtman, C. J., Jones, D. C., Kitin, P., Korripally, P. and Hammel, K. E. (2013), Spatial mapping of extracellular oxidant production by a white rot basidiomycete on wood reveals details of ligninolytic mechanism. Environmental Microbiology, 15: 956–966. doi: 10.1111/1462-2920.12039
- Issue online: 4 MAR 2013
- Version of Record online: 4 DEC 2012
- Accepted manuscript online: 9 NOV 2012 04:56AM EST
- Manuscript Accepted: 1 NOV 2012
- Manuscript Received: 26 OCT 2012
- U.S. Department of Energy, Office of Biological and Environmental Research. Grant Numbers: DE-AI02-07ER64491, DE-SC0006929
Fig. S1. a. Images showing changes in bead fluorescence after various extents of oxidation by P. chrysosporium on a wood section. From left to right, the RG ratios are 2.6, 1.5, and 1.0; and the corresponding concentrations of AAPH-derived radicals from the standard curve are 1, 10, and 25 mM. Each image frame is 5.8 μm wide.
b. Calibration curve relating RG ratios of BODIPY beads to concentrations of radicals generated by AAPH thermolysis. The points with error bars indicate experimental results with 95% confidence intervals, and the plotted curve is an empirical fit to the data using the equation shown.
Fig. S2. Relationship of predicted gradients in oxidant concentration to values of θ from the reaction–diffusion model.
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