The first two authors made an equal contribution to the work.
The peptide chain release factor methyltransferase PrmC is essential for pathogenicity and environmental adaptation of Pseudomonas aeruginosa PA14
Article first published online: 28 DEC 2012
© 2012 Society for Applied Microbiology and Blackwell Publishing Ltd
Special Issue: Environmental Ecology of Pathogens and Resistances
Volume 15, Issue 2, pages 597–609, February 2013
How to Cite
Pustelny, C., Brouwer, S., Müsken, M., Bielecka, A., Dötsch, A., Nimtz, M. and Häussler, S. (2013), The peptide chain release factor methyltransferase PrmC is essential for pathogenicity and environmental adaptation of Pseudomonas aeruginosa PA14. Environmental Microbiology, 15: 597–609. doi: 10.1111/1462-2920.12040
- Issue published online: 28 JAN 2013
- Article first published online: 28 DEC 2012
- Accepted manuscript online: 9 NOV 2012 04:55AM EST
- Manuscript Accepted: 2 NOV 2012
- Manuscript Received: 10 MAY 2012
- ERC. Grant Number: RESISTOME 
- Helmholtz International Graduate School for Infection Research. Grant Number: VH-GS-202
Pseudomonas aeruginosa pathogenicity and its capability to adapt to multiple environments are dependent on the production of diverse virulence factors, controlled by the sophisticated quorum sensing (QS) network of P. aeruginosa. To better understand the molecular mechanisms that underlie this adaptation we searched for novel key regulators of virulence factor production by screening a PA14 transposon mutant library for potential candidates acting downstream of the unique 2-alkyl-4-quinolone (AQ) QS system of P. aeruginosa. We focused the work on a protein named HemK with high homology to PrmC of Escherichia coli displaying a similar enzymatic activity (therefore also referred to as PrmC). In this study, we demonstrate that PrmC is an S-adenosyl-l-methionine (AdoMet)-dependent methyltransferase of peptide chain release factors (RFs) essential for the expression of several virulence factors, such as pyocyanin, rhamnolipids and the type III-secreted toxin ExoT. Furthermore, the PA14_prmC mutant strain is unable to grow under anoxic conditions and has a significantly reduced pathogenicity in the infection model Galleria mellonella. Along with transcriptomic and proteomic analyses, the presented data indicate that the methylation of RFs in P. aeruginosa seems to have a global effect on cellular processes related to the virulence of this nosocomial pathogen.