Characterization of Melioribacter roseus gen. nov., sp. nov., a novel facultatively anaerobic thermophilic cellulolytic bacterium from the class Ignavibacteria, and a proposal of a novel bacterial phylum Ignavibacteriae
Article first published online: 9 JAN 2013
© 2012 Society for Applied Microbiology and Blackwell Publishing Ltd
Volume 15, Issue 6, pages 1759–1771, June 2013
How to Cite
Podosokorskaya, O. A., Kadnikov, V. V., Gavrilov, S. N., Mardanov, A. V., Merkel, A. Y., Karnachuk, O. V., Ravin, N. V., Bonch-Osmolovskaya, E. A. and Kublanov, I. V. (2013), Characterization of Melioribacter roseus gen. nov., sp. nov., a novel facultatively anaerobic thermophilic cellulolytic bacterium from the class Ignavibacteria, and a proposal of a novel bacterial phylum Ignavibacteriae. Environmental Microbiology, 15: 1759–1771. doi: 10.1111/1462-2920.12067
- Issue published online: 4 JUN 2013
- Article first published online: 9 JAN 2013
- Accepted manuscript online: 11 DEC 2012 07:27AM EST
- Manuscript Accepted: 28 NOV 2012
- Manuscript Received: 16 MAY 2012
- Federal Ministry of Education and Science. Grant Number: #16.512.11.2152
- Russian Academy of Sciences
- Russian Foundation for Basic Research. Grant Number: 12-04-90841-mol_rf_nr
Table S1. Cellular fatty acid composition of strain P3M-2T. Fatty acids with content of less than 1% are not shown.
Table S2. Regression coefficient (R2), amplification efficiency, y-intercept and slope values of the standard curves for qPCR amplification of ignavibacterial and total bacterial SSU rRNA genes.
Table S3. Quantification of the ignavibacterial and total bacterial SSU rRNA.
Table S4. Characteristics of strain P3M-2T and I. album, strain Mat9-16T
Table S5. First 98 Best Blast Hits of complete 16S rRNA gene sequence of strain P3M-2T (on 13.04.2012).
Fig. S1. 23S rRNA (A) and RecA (B, amino acid sequences) sequence-based phylogenetic position of M. roseus P3M-2T. Evolutionary history was inferred using Maximal Likelihood method (NJ trees had similar topologies). Bootstrap values for 1000 replicates are shown next to the branches. Borrelia turicatae 91E135 (Spirochaetes) was chosen as an out-group.
Fig. S2. Polar lipid profile of strain P3M-2T.
Fig. S3. Precipitates of P3M-2T cells grown in aerobic conditions.
Fig. S4. Ultrathin sections of cells contrasted with ruthenium red, bar 1 μm.
Fig. S5. Extinction of acetone-methanol-extracted cells lysates. Peaks of 467, 492 and 523 nm indicated by arrows.
Fig. S6. Formation of orpiment as a result of arsenate reduction by strain P3M-2T.
Fig. S7. 16S rRNA gene-based phylogenetic position of Melioribacter roseus strain P3M-2T. Evolutionary history was inferred using Maximal Likelihood method. The percentages of 1000 bootstraps are indicated at the branching points. Borrelia turicatae 91E135 (Spirochaetes) was chosen as an out-group. Aerobic and facultatively anaerobic heterothrophs are in the red sphere. Phototrophs are in the green sphere.
Fig. S8. Source of isolation of strain P3M-2T. Bar, 5 cm.
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