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Supplemental methods.

Fig. S1. (A) Percent root length colonized and (B) percent arbuscules detected on P. lanceolata roots in the planted chamber for the AMF Permitted (closed circles) and AMF Excluded treatments (open circles). Error bars represent standard error (± SE, n = 5). Letters indicate significant differences between the time points and treatments by Bonferroni post-hoc analysis.

Fig. S2. Carbon to nitrogen (C : N) ratio of host plant material over the course of the experiment where the root-associated AMF was either permitted (filled squares, solid lines) or denied access (open squares, dashed lines) to the soil chamber containing decomposing litter. Error bars represent standard error (± SE, n = 5). Letters indicate significant differences between the time points and treatments by Bonferroni post-hoc analysis.

Table S1. Richness of all taxa detected by 16S microarray analysis in this study grouped by phyla. See Experimental procedures for presence–absence criteria.

Table S2. Richness of the bacteria and archaea that significantly increased or decreased in relative abundance (RA) in response to AMF (increased RA = + RA; decreased RA = − RA). Taxa are grouped by taxonomic class. The single archaeal taxon that responded to AMF is denoted by (A). Increases and decreases in relative abundance were calculated in the following manner: Avg. RAAMF-permitted − Avg. RAAMF-excluded. The statistical significance of these changes was determined by indicator species analysis (ISA).

Table S3. Richness of the taxa that significantly increased or decreased in relative abundance (RA) in response to AMF. Taxa are grouped by taxonomic family. Taxa are bacteria except when denoted by (A) for archaea.

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