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emi12093-sup-0001-si.tiff66K

Fig. S1. Schematic detailing construction of V. parahaemolyticus single, in frame deletion mutants. PCR 1 and PCR 2 were combined and used as a template to generate PCR 3 with the 21 bp insert. Fragment from PCR 3 was cloned into the vector pGTR1129. Plasmids with the in vitro altered sequences were transformed into E. coli SM10λpir and introduced into the wild-type V. parahaemolyticus RIMD2210633 by conjugation.

emi12093-sup-0002-si.docx79K

Table S1. Oligonucleotides used in this study.

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