Vibrio parahaemolyticus type IV pili mediate interactions with diatom-derived chitin and point to an unexplored mechanism of environmental persistence
Article first published online: 27 FEB 2013
Published 2013. This article is a U.S. Government work and is in the public domain in the USA
Special Issue: Marine Microbial Ecophysiology and Metagenomics
Volume 15, Issue 5, pages 1416–1427, May 2013
How to Cite
Frischkorn, K. R., Stojanovski, A. and Paranjpye, R. (2013), Vibrio parahaemolyticus type IV pili mediate interactions with diatom-derived chitin and point to an unexplored mechanism of environmental persistence. Environmental Microbiology, 15: 1416–1427. doi: 10.1111/1462-2920.12093
- Issue published online: 18 APR 2013
- Article first published online: 27 FEB 2013
- Accepted manuscript online: 24 JAN 2013 12:25PM EST
- Manuscript Revised: 17 JAN 2013
- Manuscript Accepted: 17 JAN 2013
- Manuscript Received: 14 NOV 2012
- NOAA Oceans and Human Health Initiative
Fig. S1. Schematic detailing construction of V. parahaemolyticus single, in frame deletion mutants. PCR 1 and PCR 2 were combined and used as a template to generate PCR 3 with the 21 bp insert. Fragment from PCR 3 was cloned into the vector pGTR1129. Plasmids with the in vitro altered sequences were transformed into E. coli SM10λpir and introduced into the wild-type V. parahaemolyticus RIMD2210633 by conjugation.
Table S1. Oligonucleotides used in this study.
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