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Supplementary methods.

Table S1. Data flow overview.

Fig. S1. Length distribution of experimentally confirmed signal peptides from E. coli K-12 (the UniProt set). Dotted lines denote the length range covering at least 90% of all peptides.

Table S2. The list of 129 E. coli K-12 N-terminal cleaved peptide sequences detected by proteogenomics, except for N-terminal methionine excision.

Table S3. The list of identified 96 putative signal peptides.

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