L.B. and L.V. contributed equally to this work.
Linking isoprenoidal GDGT membrane lipid distributions with gene abundances of ammonia-oxidizing Thaumarchaeota and uncultured crenarchaeotal groups in the water column of a tropical lake (Lake Challa, East Africa)
Article first published online: 8 APR 2013
© 2013 John Wiley & Sons Ltd and Society for Applied Microbiology
Special Issue: Drivers of Shifts in Microbial Community Composition
Volume 15, Issue 9, pages 2445–2462, September 2013
How to Cite
Buckles, L. K., Villanueva, L., Weijers, J. W. H., Verschuren, D. and Damsté, J. S. S. (2013), Linking isoprenoidal GDGT membrane lipid distributions with gene abundances of ammonia-oxidizing Thaumarchaeota and uncultured crenarchaeotal groups in the water column of a tropical lake (Lake Challa, East Africa). Environmental Microbiology, 15: 2445–2462. doi: 10.1111/1462-2920.12118
- Issue published online: 4 SEP 2013
- Article first published online: 8 APR 2013
- Accepted manuscript online: 14 MAR 2013 12:52AM EST
- Manuscript Accepted: 7 MAR 2013
- Manuscript Received: 29 NOV 2012
- European Research Council. Grant Numbers: FP7/2007–2013, 226600
Stratified lakes are important reservoirs of microbial diversity and provide habitats for niche differentiation of Archaea. In this study, we used a lipid biomarker/DNA-based approach to reveal the diversity and abundance of Archaea in the water column of Lake Challa (East Africa). Concentrations of intact polar lipid (IPL) crenarchaeol, a specific biomarker of Thaumarchaeota, were enhanced (1 ng l−1) at the oxycline/nitrocline. The predominance of the more labile IPL hexose-phosphohexose crenarchaeol indicated the presence of an actively living community of Thaumarchaeota. Archaeal 16S rRNA clone libraries revealed the presence of thaumarchaeotal groups 1.1a and 1.1b at and above the oxycline. In the anoxic deep water, amoA gene abundance was an order of magnitude lower than at the oxycline and high abundance (∼ 90 ng l−1) of an IPL with the acyclic glycerol dialkyl glycerol tetraether (GDGT-0) was evident. The predominance of archaeal 16S rRNA sequences affiliated to the uncultured crenarchaeota groups 1.2 and miscellaneous crenarchaeotic group (MCG) points to an origin of GDGT-0 from uncultured crenarchaeota. This study demonstrates the importance of thermal stratification and nutrient availability in the distribution of archaeal groups in lakes, which is relevant to constrain and validate temperature proxies based on archaeal GDGTs (i.e. TEX86).