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emi12159-sup-0001-FigS1.eps1655K

Figure S1. Maximum likelihood tree of representative sequences from dominant OTUs in the NIC enrichments MCC and wheat arabinoxylan as uncovered in (1) Sanger-based passage 4 DNA and cDNA clone libraries at passage 4, (2) SSU amplicon sequencing of passage 4 (described as 454_OTU97_#) and (3) high-resolution of MCC-adapted thermophilic passage (described as TC_OTU97_#). The tree is based on the 16S rRNA gene using sequences (ca. 800 bp) obtained from and environmental clones. Small SSU amplicon sequences (454_OTU97_#) were quick added to the tree with parsimony.

emi12159-sup-0002-FigS2.docx54K

Figure S2. Differential endoglucanase activity for thermophilic consortia adapted to microcrystalline cellulose (mcc) and wheat arabinoxylan (xylan) on filter sterilized (0.2 μm filter) supernatant (fs), unfiltered whole cells (wc) and sonicated cells (son).

emi12159-sup-0003-FigS3.eps1840K

Figure S3. Glycosyl hydrolase activities during the establishment of the thermophilic, microcrystalline cellulose Picture depicts the colorometric changes noted during the development of this consortia.

emi12159-sup-0004-FigS4.eps1263K

Figure S4. Relationship between the relative abundance of Bacteroidetes (A), Firmicutes (B) and Thermus (C) based on SSU amplicon libraries and CrI using X-ray diffraction. Starting crystallinity of the cellulose prior to microbial transformation is depicted with an arrow in each panel.

emi12159-sup-0005-FigS5.eps1141K

Figure S5. Experimental design of the establishment for the thermophilic consortia (A) and high-resolution analysis of the MCC-adapted thermophilic consortia within a passage (B).

emi12159-sup-0006-TableS1.docx48K

Table S1. Average DNA (μg) per 50 ml of culture ± standard deviation (n = 3 biological replicates).

emi12159-sup-0007-TableS2.docx133K

Table S2. Relative proportion clusters (OTU97, > 1%) for passage 4 microcrystalline cellulose (MCC) and wheat arabinoxylan (xylan). Phylum description was assigned by the Ribosomal Database Project Classifier, Release 10. The OTU identification was assigned by GenBank.

emi12159-sup-0008-TableS3.docx66K

Table S3. Average endoglucanase and endoxylanase activities (U ml–1 min–1) ± standard deviation (n = 3 biological replicates) measured in whole cell fractions across the five passages for the various substrates at 65°C, pH = 6.5. ND = not detected.

emi12159-sup-0009-TableS4.docx51K

Table S4. Physical and chemical properties the Newby Island compost inoculum used in the thermophilic enrichments. ΔSoil testing was performed at New Mexico State University Soil, Water, and Agricultural Testing Lab (Las Cruces, NM).

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