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emi12164-sup-0001-fs1.tif1694K

Fig. S1. Growth (OD600) of P. aeruginosa PAO1 (circles), PAO1 ΔrsmA (squares) and PAO1 ΔrsmY ΔrsmZ (diamonds) at 37°C in TSBD medium. Values represent the mean (± standard deviation) of at least three independent assays.

emi12164-sup-0002-fs2.tif2667K

Fig. S2. Activity of (A) PpvdS’-‘lacZ and (B) PpchR’-‘lacZ translational fusions in P. aeruginosa PAO1 (black bars), PAO1 ΔrsmA (white bars) and PAO1 ΔrsmY ΔrsmZ (grey bars) grown in TSBD medium at 37°C. Values are the mean (± standard deviation) of three independent assays.

emi12164-sup-0003-fs3.tif1508K

Fig. S3. Activity of the PfeoA::lacZ translational fusions in P. aeruginosa PAO1, PAO1 ΔrsmA and PAO1 ΔrsmY ΔrsmZ grown for 14 h in TSBD medium at 37°C. Values are the mean (± standard deviation) of three independent experiments performed in duplicate.

emi12164-sup-0004-fs4.tif1418K

Fig. S4. Activity of the PA4492'-‘lacZ translational fusions in P. aeruginosa PAO1, PAO1 ΔrsmA and PAO1 ΔrsmY ΔrsmZ grown for 8 h in TSBD medium at 37°C. Values are the mean (± standard deviation) of three independent assays.

emi12164-sup-0005-fs5.tif1684K

Fig. S5. Activity of the PpvdS::lacZ translational fusions in P. aeruginosa PAO1 (black bars), PAO1 ΔrsmA (white bars) and PAO1 ΔrsmY ΔrsmZ (grey bars) harbouring a plasmid that constitutively expresses the diguanylate cyclase WspRR242A (wspRR242A) or the empty plasmid (ctl), grown for 14 h in TSBD at 37°C. Results are the mean (± standard deviation) of four independent assays.

emi12164-sup-0006-ts1.doc72K

Table S1. Bacterial strains and plasmids used in this study.

emi12164-sup-0007-ts2.doc33K

Table S2. Primers used in this study1.

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