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emi12187-sup-0001-si.m4v26544K

Supplemental Movie S1. FIB/SEM 3D volume of M. xanthus biofilm. Slice-by-slice inspection of a large 3D volume of high-pressure frozen, freeze-substituted and resin-embedded M. xanthus biofilm volume, revealing the presence of a network virtually connecting all bacterial community member with one another. Note that each cell displays multiple connections to neighbouring cells.

emi12187-sup-0002-si.mov16772K

Supplemental Movie S2. 3D rendering of 3D FIB/SEM 3D volume of M. xanthus biofilm. 3D rendering provides a comprehensive view on the extent of the intercellular connections that link the cells in the biofilm.

emi12187-sup-0003-si.mov14806K

Supplemental Movie S3. TEM tomogram of constricted vesicle chains in M. xanthus biofilms. Slice-by-slice inspection of a 3D tomographic volume of high-pressure frozen, freeze-substituted and resin-embedded 150 nm thin M. xanthus biofilm section, revealing the presence of periodically constricted vesicle chains/membrane tubes, displaying a continuous volume.

emi12187-sup-0004-si.m4v8396K

Supplemental Movie S4. Cryo-TEM tomogram of vesicle chains. Slice-by-slice inspection of a 3D tomographic volume of frozen-hydrated vesicle chains present in M. xanthus planktonic cell suspensions. Note that within one chain, all vesicles display the same diameter, whereas vesicle diameter varies significantly from vesicle chain to vesicle chain.

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