Bacterial social networks: structure and composition of Myxococcus xanthus outer membrane vesicle chains
Article first published online: 15 JUL 2013
© 2013 Society for Applied Microbiology and John Wiley & Sons Ltd
Thematic Issue: Protists
Volume 16, Issue 2, pages 598–610, February 2014
How to Cite
Remis, J. P., Wei, D., Gorur, A., Zemla, M., Haraga, J., Allen, S., Witkowska, H. E., Costerton, J. W., Berleman, J. E. and Auer, M. (2014), Bacterial social networks: structure and composition of Myxococcus xanthus outer membrane vesicle chains. Environmental Microbiology, 16: 598–610. doi: 10.1111/1462-2920.12187
- Issue published online: 27 JAN 2014
- Article first published online: 15 JUL 2013
- Accepted manuscript online: 18 JUN 2013 06:36AM EST
- Manuscript Accepted: 8 JUN 2013
- Manuscript Received: 3 JUN 2013
- Office of Biological and Environmental Research of the US Department of Energy. Grant Number: DE-AC02-05CH11231
Supplemental Movie S1. FIB/SEM 3D volume of M. xanthus biofilm. Slice-by-slice inspection of a large 3D volume of high-pressure frozen, freeze-substituted and resin-embedded M. xanthus biofilm volume, revealing the presence of a network virtually connecting all bacterial community member with one another. Note that each cell displays multiple connections to neighbouring cells.
Supplemental Movie S2. 3D rendering of 3D FIB/SEM 3D volume of M. xanthus biofilm. 3D rendering provides a comprehensive view on the extent of the intercellular connections that link the cells in the biofilm.
Supplemental Movie S3. TEM tomogram of constricted vesicle chains in M. xanthus biofilms. Slice-by-slice inspection of a 3D tomographic volume of high-pressure frozen, freeze-substituted and resin-embedded 150 nm thin M. xanthus biofilm section, revealing the presence of periodically constricted vesicle chains/membrane tubes, displaying a continuous volume.
Supplemental Movie S4. Cryo-TEM tomogram of vesicle chains. Slice-by-slice inspection of a 3D tomographic volume of frozen-hydrated vesicle chains present in M. xanthus planktonic cell suspensions. Note that within one chain, all vesicles display the same diameter, whereas vesicle diameter varies significantly from vesicle chain to vesicle chain.
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