A morphogenetic survey on ciliate plankton from a mountain lake pinpoints the necessity of lineage-specific barcode markers in microbial ecology
Article first published online: 15 JUL 2013
© 2013 The Authors. Environmental Microbiology published by Society for Applied Microbiology and John Wiley & Sons Ltd.
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Thematic Issue: Protists
Volume 16, Issue 2, pages 430–444, February 2014
How to Cite
Stoeck, T., Breiner, H.-W., Filker, S., Ostermaier, V., Kammerlander, B. and Sonntag, B. (2014), A morphogenetic survey on ciliate plankton from a mountain lake pinpoints the necessity of lineage-specific barcode markers in microbial ecology. Environmental Microbiology, 16: 430–444. doi: 10.1111/1462-2920.12194
- Issue published online: 27 JAN 2014
- Article first published online: 15 JUL 2013
- Accepted manuscript online: 19 JUN 2013 06:38AM EST
- Manuscript Revised: 11 JUN 2013
- Manuscript Accepted: 11 JUN 2013
- Manuscript Received: 9 APR 2013
- Austrian Science Fund (FWF). Grant Numbers: P21013-B03, P16559-B06
- Deutsche Forschungsgemeinschaft (DFG). Grant Number: STO414/3-2
Fig. S1. Rank-abundance distribution of amplicon data sets from the three different depths screened for ciliate phylotype diversity in Piburgersee in March 2011. The distribution follows a long-tail distribution, typically for large environmental amplicon data sets.
Fig. S2. Log-regression of relative abundances of amplicon ciliate genera and morphotype ciliate genera. Abundance distributions are incongruent and do not correlate, suggesting that amplicon read abundances do not reflect morphotaxon (organismic) abundances.
Table S1. ‘Candidatus ciliate genera’ that were detected through V4 SSU rDNA amplicons in data sets from the three different depths screened for ciliate phylotype diversity in Piburgersee in March 2011. Amplicons were assigned to a ‘candidatus genus’ status when the maximum sequence similarity to a reference sequence database of described ciliate morphotypes did not exceed 95%.
Table S2. Ciliate genera and number of species (in brackets) detected in Piburgersee on 1 March, 2011 in order of relative frequency (mean abundance in ind. l−1 over the three sampling depths) and abundance in the respective depth. n = 6 (0 m, 9 m), n = 3 (21 m), n.d., not detected.
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