Diversity and distribution of a key sulpholipid biosynthetic gene in marine microbial assemblages
Article first published online: 24 JUL 2013
© 2013 John Wiley & Sons Ltd and Society for Applied Microbiology
Volume 16, Issue 3, pages 774–787, March 2014
How to Cite
Villanueva, L., Bale, N., Hopmans, E. C., Schouten, S. and Damsté, J. S. S. (2014), Diversity and distribution of a key sulpholipid biosynthetic gene in marine microbial assemblages. Environmental Microbiology, 16: 774–787. doi: 10.1111/1462-2920.12202
- Issue published online: 3 MAR 2014
- Article first published online: 24 JUL 2013
- Accepted manuscript online: 26 JUN 2013 04:40AM EST
- Manuscript Accepted: 22 JUN 2013
- Manuscript Revised: 31 MAY 2013
- Manuscript Received: 5 DEC 2012
- Division of Earth and Life Sciences of the Dutch Organization. Grant Number: 839.08.331
Fig. S1. Details of the SQDG biosynthetic pathway.
Fig. S2. Phylogenetic clustering of putative UDP-SQ synthase (sqdB gene-coding protein) included in Fig. 1 and the closely related metagenomic sequences (pBLAST e-value 1e−50 with marine microbiome metagenomes included in IMG/M). Number code (1–18) corresponds to the metagenome project as indicated in Table S2. The phylogenetic tree was inferred by maximum likelihood with the LG + G + I model of protein evolution. Branch support was calculated with the approximate likelihood ratio test (aLRT) and indicated on the branches if > 50%. This is a topographic representation, and the branches are not proportional to the evolutionary distance between sequences.
Fig. S3. Details of aLRT branch support of cluster 1 cyanobacteria in Fig. 1.
Table S1. Bacterial and microalgae species in which sulphoquinovosyldiacylglycerols (SQDGs) have been reported.
Table S2. Metagenome projects from marine microbiomes in IMG/M used in this study.
Table S3. Primers for sqdB gene sequences of the phylum Cyanobacteria (clusters sqdB1A–B, and sqdB2A), and α-Rhodobacterales and α-Rhizobiales groups (cluster sqdB2B).
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