The copyright line of this article has been changed on 29 May 2014, since its first online publication.
Interference with the germination and growth of Ulva zoospores by quorum-sensing molecules from Ulva-associated epiphytic bacteria
Version of Record online: 23 JUL 2013
© 2013 The Authors. Environmental Microbiology published by Society for Applied Microbiology and John Wiley & Sons Ltd
This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
Thematic Issue: Protists
Volume 16, Issue 2, pages 445–453, February 2014
How to Cite
Twigg, M. S., Tait, K., Williams, P., Atkinson, S. and Cámara, M. (2014), Interference with the germination and growth of Ulva zoospores by quorum-sensing molecules from Ulva-associated epiphytic bacteria. Environmental Microbiology, 16: 445–453. doi: 10.1111/1462-2920.12203
- Issue online: 27 JAN 2014
- Version of Record online: 23 JUL 2013
- Accepted manuscript online: 26 JUN 2013 04:42AM EST
- Manuscript Accepted: 22 JUN 2013
- Manuscript Received: 5 MAR 2013
- UK Natural Environment Research Council. Grant Number: NE/F012365/1
Fig. S1. Phylogenetic tree of the Ulva thallus bacterial population. The tree resulted from a sequence alignment of 16S rDNA from bacterial clones and isolates obtained from the Ulva thallus at 97% sequence similarity. The reference strains were taken from GenBank. In brackets is the number of clones in each OTU. The tree topology is based on neighbour- joining and bootstrap analysis was performed with 1000 replications.
Fig. S2. AHL bioreporter activation assay.
A. Cell-free supernatant extracts from Shewanella spp. 79 pBBRIMCS and pMT01 were assayed with lux-based AHL bioreporters E. coli pSB536 (clear bars) and pSB1075 (cross-hatched bars).
B. Cell-free supernatant extracts from Sulfitobacter spp. 376 pBBRIMCS and pMT01 were assayed with lux-based AHL bioreporter E. coli pSB401. All strains harbouring pMT01 and therefore expressing the aiiA lactonase failed to activate the bioreporters showing that their cognate AHLs were being deactivated.
Fig. S3. AHL germination assays slides. Example images of Ulva zoospores germinated on (A) Sulfitobacter sp. pMT01 biofilms (AHL-deficient) and (B) Sulfitobacter sp. pBBRIMCS biofilms (AHL-producing). Bar = 50 μm.
Table S1. Phylogenetic identification and AHL signal molecule production in Ulva-associated marine bacteria. The ability of each strain to produce AHLs was assayed using lux-based AHL bioreporters, a positive result is indicated by + and a negative result by –. AHL production and identity was confirmed by LC-MS/MS.
Table S2. Non-marine bacterial strains used in this study.
Table S3. Plasmids used in this study.
Please note: Wiley Blackwell is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.