Stationary phase and mature infectious forms of Legionella pneumophila produce distinct viable but non-culturable cells
Version of Record online: 22 AUG 2013
© 2013 Society for Applied Microbiology and John Wiley & Sons Ltd
Thematic Issue: Protists
Volume 16, Issue 2, pages 382–395, February 2014
How to Cite
Al-Bana, B. H., Haddad, M. T. and Garduño, R. A. (2014), Stationary phase and mature infectious forms of Legionella pneumophila produce distinct viable but non-culturable cells. Environmental Microbiology, 16: 382–395. doi: 10.1111/1462-2920.12219
- Issue online: 27 JAN 2014
- Version of Record online: 22 AUG 2013
- Accepted manuscript online: 25 JUL 2013 04:09AM EST
- Manuscript Revised: 15 JUL 2013
- Manuscript Accepted: 15 JUL 2013
- Manuscript Received: 17 MAR 2013
- Canadian Institutes of Health Research
- Nova Scotia Health Research Foundation
- Iraqi Ministry of High Education and Scientific Research
Fig. S1. The viability trendline of VBNCCs reaches an asymptote. Viability of SPF-VBNCCs in tap water at 45°C was followed for up to 101 days (60 days after SPFs entered the VBNC state) and graphed as % viability versus time. A trendline was drawn (broken grey line) that becomes asymptotic at ∼13% viability. Dots show the mean of three independent measures, but error bars were omitted for clarity.
Table S1. Concentration of the ions added to dd-water buffered to pH 6.6 to test their effect on SPFs culturability at 45°C. Ion concentration was obtained from the Halifax Regional Water Commission (HRWC) 2005/06 water quality report (Halifax Regional Water Commission, 2006).
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