Fig. S1. The viability trendline of VBNCCs reaches an asymptote. Viability of SPF-VBNCCs in tap water at 45°C was followed for up to 101 days (60 days after SPFs entered the VBNC state) and graphed as % viability versus time. A trendline was drawn (broken grey line) that becomes asymptotic at ∼13% viability. Dots show the mean of three independent measures, but error bars were omitted for clarity.


Table S1. Concentration of the ions added to dd-water buffered to pH 6.6 to test their effect on SPFs culturability at 45°C. Ion concentration was obtained from the Halifax Regional Water Commission (HRWC) 2005/06 water quality report (Halifax Regional Water Commission, 2006).

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