Rare earth metals are essential for methanotrophic life in volcanic mudpots
Article first published online: 12 SEP 2013
© 2013 Society for Applied Microbiology and John Wiley & Sons Ltd
Special Issue: Metabolism & Biodegradation
Volume 16, Issue 1, pages 255–264, January 2014
How to Cite
Pol, A., Barends, T. R. M., Dietl, A., Khadem, A. F., Eygensteyn, J., Jetten, M. S. M. and Op den Camp, H. J. M. (2014), Rare earth metals are essential for methanotrophic life in volcanic mudpots. Environmental Microbiology, 16: 255–264. doi: 10.1111/1462-2920.12249
- Issue published online: 6 JAN 2014
- Article first published online: 12 SEP 2013
- Accepted manuscript online: 18 AUG 2013 10:33PM EST
- Manuscript Accepted: 9 AUG 2013
- Manuscript Revised: 6 AUG 2013
- Manuscript Received: 10 JUN 2013
- Mosaic. Grant Number: 017.005.113
- Netherlands Organization for Scientific Research
- European Research Council. Grant Number: 232937
- Max-Planck Society
Fig. S1. Uptake preference within the series of REE (La, Ce, Pr, Nd) during growth in MPW-supplemented (4%) medium.
Fig. S2. SDS-PAGE (12%) of the purified methanol dehydrogenase of strain SolV (A) and the UV-Vis spectrum of the pure enzyme (B).
Fig. S3. Analytical ultracentrifugation analysis of purified MDH.
Fig. S4. Effect of pH on MDH activity. Activities refer to the μM DCPIP reduced per min. Each symbol represents an individual measurement. Squares and triangles indicate measurements with and without 40 mM ammonium chloride respectively.
Fig. S5. A. Kinetics of purified MDH. Symbols indicate individual rate measurements. Lines represent the curves obtained by fitting Michaelis–Menten kinetics. Obtained affinity constants (Km) are given in Table 3.
B. Kinetics of purified MDH. Symbols indicate individual rate measurements. Lines represent the curves obtained by fitting Michaelis–Menten kinetics. Obtained affinity constants (Km) are given in Table 3.
Table S1. Crystallographic data and model quality.
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