Fig. S1. Southern blot analyses of the Δlaccase mutants. DNA was isolated from wild-type P. anserina and one or several purified transformants (T1, T2) and digested with appropriate restriction enzymes. The blots were probed either with a sequence containing the relevant CDS and its flanking region. For each laccase gene, a restriction map of the wild-type and mutant locus is presented. The sizes of the expected fragments are indicated on the maps and are reported close to the corresponding fragment on the Southern blot.

Table S1. Primers for gene deletion and detection.

Table S2. Plasmids for complementation of the mutants.

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