Systematic gene deletions evidences that laccases are involved in several stages of wood degradation in the filamentous fungus Podospora anserina
Version of Record online: 18 SEP 2013
© 2013 Society for Applied Microbiology and John Wiley & Sons Ltd
Special Issue: Metabolism & Biodegradation
Volume 16, Issue 1, pages 141–161, January 2014
How to Cite
Xie, N., Chapeland-Leclerc, F., Silar, P. and Ruprich-Robert, G. (2014), Systematic gene deletions evidences that laccases are involved in several stages of wood degradation in the filamentous fungus Podospora anserina. Environmental Microbiology, 16: 141–161. doi: 10.1111/1462-2920.12253
- Issue online: 6 JAN 2014
- Version of Record online: 18 SEP 2013
- Accepted manuscript online: 23 AUG 2013 04:00AM EST
- Manuscript Accepted: 11 AUG 2013
- Manuscript Revised: 22 JUL 2013
- Manuscript Received: 3 MAY 2013
Fig. S1. Southern blot analyses of the Δlaccase mutants. DNA was isolated from wild-type P. anserina and one or several purified transformants (T1, T2) and digested with appropriate restriction enzymes. The blots were probed either with a sequence containing the relevant CDS and its flanking region. For each laccase gene, a restriction map of the wild-type and mutant locus is presented. The sizes of the expected fragments are indicated on the maps and are reported close to the corresponding fragment on the Southern blot.
Table S1. Primers for gene deletion and detection.
Table S2. Plasmids for complementation of the mutants.
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