These authors contributed equally to this work.
Seasonally variable intestinal metagenomes of the red palm weevil (Rhynchophorus ferrugineus)
Article first published online: 9 OCT 2013
© 2013 Society for Applied Microbiology and John Wiley & Sons Ltd
Thematic Issue: Invertebrate–Microbe Interactions
Volume 15, Issue 11, pages 3020–3029, November 2013
How to Cite
Jia, S., Zhang, X., Zhang, G., Yin, A., Zhang, S., Li, F., Wang, L., Zhao, D., Yun, Q., Tala, Wang, J., Sun, G., Baabdullah, M., Yu, X., Hu, S., Al-Mssallem, I. S. and Yu, J. (2013), Seasonally variable intestinal metagenomes of the red palm weevil (Rhynchophorus ferrugineus). Environmental Microbiology, 15: 3020–3029. doi: 10.1111/1462-2920.12262
- Issue published online: 25 OCT 2013
- Article first published online: 9 OCT 2013
- Accepted manuscript online: 30 AUG 2013 04:48AM EST
- Manuscript Accepted: 23 AUG 2013
- Manuscript Revised: 26 JUN 2013
- Manuscript Received: 10 OCT 2012
- KACST, Riyadh, Saudi Arabia. Grant Number: 428-29
The intestinal microbes residing in the red palm weevil (RPW, Rhynchophorus ferrugineus) larva consume tender interior fibrous tissues of date palm trunks. The understanding of such microbiota at molecular level provides vital clues for the biological control of this devastating pest. Using pyrosequencing and shotgun strategy, we first study taxonomic profiles of the microbiota sampled at different months (March, July and November), and then confirm the impact of high-temperature stress on the microbial populations based on data from 16S rRNA amplicons using both field and laboratory samples. We further identify Klebsiella pneumoniae in November and Lactococcus lactis in July as the dominant species of the microbiota. We find that the RPW gut microbiota degrades polysaccharides and sucrose with hydrolases and that different active bacterial species in November and July are responsible for the symbiotic relationship between the microbiota and the host. Our results provide vital information for pest control and cellulolytic bacterial species characterization.