NrsZ: a novel, processed, nitrogen-dependent, small non-coding RNA that regulates Pseudomonas aeruginosa PAO1 virulence
Article first published online: 6 OCT 2013
© 2013 The Authors. Environmental Microbiology published by Society for Applied Microbiology and John Wiley & Sons Ltd.
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Special Issue: Environmental Lifestyles and Transmission of Pathogens
Volume 16, Issue 4, pages 1053–1068, April 2014
How to Cite
Wenner, N., Maes, A., Cotado-Sampayo, M. and Lapouge, K. (2014), NrsZ: a novel, processed, nitrogen-dependent, small non-coding RNA that regulates Pseudomonas aeruginosa PAO1 virulence. Environmental Microbiology, 16: 1053–1068. doi: 10.1111/1462-2920.12272
- Issue published online: 2 APR 2014
- Article first published online: 6 OCT 2013
- Accepted manuscript online: 4 SEP 2013 11:46AM EST
- Manuscript Accepted: 28 AUG 2013
- Manuscript Revised: 26 AUG 2013
- Manuscript Received: 25 APR 2013
- Sandoz Family Foundation (Programme for Academic Promotion)
- Swiss National Foundation. Grant Number: 31003A-127587
- Fondation Herbette
The opportunistic pathogen Pseudomonas aeruginosa PAO1 has a remarkable capacity to adapt to various environments and to survive with limited nutrients. Here, we report the discovery and characterization of a novel small non-coding RNA: NrsZ (nitrogen-regulated sRNA). We show that under nitrogen limitation, NrsZ is induced by the NtrB/C two-component system, an important regulator of nitrogen assimilation and P. aeruginosa's swarming motility, in concert with the alternative sigma factor RpoN. Furthermore, we demonstrate that NrsZ modulates P. aeruginosa motility by controlling the production of rhamnolipid surfactants, virulence factors notably needed for swarming motility. This regulation takes place through the post-transcriptional control of rhlA, a gene essential for rhamnolipids synthesis. Interestingly, we also observed that NrsZ is processed in three similar short modules, and that the first short module encompassing the first 60 nucleotides is sufficient for NrsZ regulatory functions.