Transcriptional profiling of Gram-positive Arthrobacter in the phyllosphere: induction of pollutant degradation genes by natural plant phenolic compounds
Article first published online: 23 JAN 2014
© 2013 Society for Applied Microbiology and John Wiley & Sons Ltd
Special Issue: Plant-Microbe Interactions
Volume 16, Issue 7, pages 2212–2225, July 2014
How to Cite
Scheublin, T. R., Deusch, S., Moreno-Forero, S. K., Müller, J. A., van der Meer, J. R. and Leveau, J. H. J. (2014), Transcriptional profiling of Gram-positive Arthrobacter in the phyllosphere: induction of pollutant degradation genes by natural plant phenolic compounds. Environmental Microbiology, 16: 2212–2225. doi: 10.1111/1462-2920.12375
- Issue published online: 2 JUL 2014
- Article first published online: 23 JAN 2014
- Accepted manuscript online: 24 DEC 2013 04:41AM EST
- Manuscript Accepted: 12 DEC 2013
- Manuscript Received: 15 NOV 2013
Fig. S1. GC-MS analysis of Phaseolus vulgaris leaf surface wash extracts. A peak with the retention time (19.6 min) of silated hydroquinone is indicated in a chromatogram section by an arrow (A). The corresponding mass spectrum (B) matched that of silated hydroquinone from the NIST Mass Spectrometry Data Center (C) and of an authentic standard (not shown).
Fig. S2. Population sizes of A. chlorophenolicus A6 on bean leaves that were sampled for RNA extraction and subsequent microarray analysis. ‘Wet’ refers to the PhylH treatment, while ‘dry’ refers to the PhylL treatment.
Table S1. Fold change in gene expression and corrected P values of A. chlorophenolicus genes on transcriptome arrays. Genes with a more than twofold change in gene expression and a corrected P value lower than 0.05 are indicated in bold. Comparisons were made between (i) high-humidity phyllosphere and agar surface treatment, (ii) low-humidity phyllosphere and agar surface treatment, and (iii) chlorophenol and agar surface treatment. Genes with a significantly different expression between high and low phyllosphere humidity treatments are indicated with asterisks (** = P < 0.05, * = P < 0.1).
Table S2. Compounds identified in three independent methanol extracts from the surface of bean leaves.
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