Autolysis detection and evaluation of some lactic acid bacteria by renaturing sodium dodecyl sulphate-polyacrylamide gel electrophoresis and polymerase chain reaction assays

Authors

  • Abdulrahman A Al-Saleh,

    1. Department of Food Science and Nutrition, King Saud University, Riyadh, Saudi Arabia
    Search for more papers by this author
  • Elsayed A Ismail,

    Corresponding author
    1. Department of Food Science and Nutrition, King Saud University, Riyadh, Saudi Arabia
    2. Faculty of Agriculture, Food Science Department, Benha University, Benha, Egypt
    Search for more papers by this author
  • Ali Am Metwalli

    1. Department of Food Science and Nutrition, King Saud University, Riyadh, Saudi Arabia
    2. Dairy Science Department, College of Agriculture, Menia University, Menia 61519, Egypt
    Search for more papers by this author

Abstract

Eleven lactic acid bacterial strains were tested for autolysis ability and the presence of autolytic enzymes by renaturing sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE). Polymerase chain reaction (PCR) assays for the detection of lysogenic strains were performed. Autolysis in a buffer system was observed in Lactobacillus delbrueckii subsp. bulgaricus DSM 20080, L. delbrueckii subsp. bulgaricus DSM 20081, Bifidobacterium longum subsp. infantis DSM 20088, B. angulatum DSM 20098 and Streptococcus thermophillus DSM 20617. Mitomycin C induction of prophage was demonstrated in B. longum subsp. infantis DSM 20088, B. angulatum DSM 20098, Lactobacillus acidophilus DSM 20242 and S. thermophillus DSM 20617. The presence of genes encoding known bacteriophage lysins was demonstrated by a PCR assay and correlated well with the autolytic phenotypes of the strains, indicating that PCR screening is useful in the rapid identification of autolytic strains.

Ancillary