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Fig. S1. Neighbour-Joining tree for FSY1 nucleotide sequences (Saitou & Nei, 1987).

Fig. S2. Multiplex ladder of individual Saccharomyces species tested with a primer-pair set of all seven species. 1 – S. cerevisiaeNT; 2 – S. eubayanusT; 3 – S. uvarum CBS 7001; 4 – S. arboricolaT ; 5 – S. mikataeT ; 6 – S. kudriavzeviiT; 7 – S. paradoxusT

Fig. S3. PCR products obtained from Seub F3/R2 at 55 °C against members of the family Saccharomycetaceae.

Fig. S4. Primers Seub F3/R2 tested against species commonly found in wine fermentations.

Fig. S5. Saccharomyces bayanus positive isolates from Fig. 4 retested with Seub F3/R2. 1 – S. eubayanusT; 2 – S. cerevisiaeNT; 3–7 – S. bayanus: 3 – CBS 380T; 4 – NCYC 374; 5 – NCYC 114 (=NBRC 1948); 6 – CBS 1505; 7 – CBS 1546.

Fig. S6. Saccharomyces complex genomes tested with Seub F3/R2.

Table S1. List of yeast species described only in the Supporting Information.

Please note: Wiley Blackwell is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.