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Fig. S1. Phylogenetic tree of sequences from clone libraries show the relationship of the sequences analyzed in the TRFLP method within the Chloroflexi.

Fig. S2. Relationship of clones obtained from qPCR amplification using the primers Chlflx1150F and Dhc1286R in this study.

Fig. S3. Principal component analysis of qPCR data, sediment characterization data, and porewater characterization data for all samples subject to ICP-AES (35 grab samples and core sections).

Table S1. Longitude and latitude of sampling location for the lakes sampled in this study.

Table S2. Summary of results from clone library analysis TRFLP primers between Dehalococcoidetes (Ddts) class and other Chloroflexi classes (Other).

Table S3. Summary table of TRFLP results for each grab sediment sample.

Table S4. Summary of data comparing the methodology for porewater ‘sulfur’ used in this study to measured porewater sulfide.

Table S5. Data collected in this study not presented elsewhere.

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