Table S1. Conditions used for quantitative PCR of group specific one primers.

Table S2. Reference sequences and associated Pester et al. (2012) phylogeny used to overlay amoA phylogenetic clusters on the global AOA tree.

Table S3. Correlation statistics for archaeal amoA gene abundances (g−1 soil and /total bacteria+archaea) and environmental variables

Fig. S1. Rarefaction analysis of unique and 98% identity sequenced amoA clones from three forest types in the Luquillo Experimental Forest, Puerto Rico: high-elevation cloud forest, mid-elevation colorado forest and low-elevation tabonuco forest.

Fig. S2. Global amoA maximum likelihood tree with branches colored by confidence scores for each split (green > 75% support, yellow > 50% support, red < 50% support).

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