Temporal changes in particle-associated microbial communities after interception by nonlethal sediment traps

Authors

  • Gary R. LeCleir,

    1. Department of Microbiology, The University of Tennessee, Knoxville, TN, USA
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  • Jennifer M. DeBruyn,

    1. Biosystems Engineering and Soil Science, The University of Tennessee, Knoxville, TN, USA
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  • Elizabeth W. Maas,

    1. National Institute of Water and Atmospheric Research, Wellington, New Zealand
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  • Philip W. Boyd,

    1. Department of Chemistry, NIWA Centre for Chemical and Physical Oceanography, University of Otago, Dunedin, New Zealand
    2. Institute for Marine and Antarctic Studies, University of Tasmania, Hobart, Tasmania Australia
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  • Steven W. Wilhelm

    Corresponding author
    1. Department of Microbiology, The University of Tennessee, Knoxville, TN, USA
    • Correspondence: Steven W. Wilhelm, Department of Microbiology, The University of Tennessee, Knoxville, TN 37996, USA. Tel.: +1 865 974 0665; fax: +1 865 974 4007; e-mail: wilhelm@utk.edu

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Abstract

Using marine sediment traps (named RESPIRE for REspiration of Sinking Particles In the subsuRface ocEan) designed to collect sinking particles and associated microbial communities in situ, we collected and incubated marine aggregates/particles in the southern Pacific Ocean from separate phytoplankton bloom events in situ. We determined the phylogenetic affiliation for the microorganisms growing on aggregates by pyrosequencing partial 16S rRNA gene amplicons. Water column samples were also collected and sequenced for comparison between sinking-particle-associated and planktonic bacterial communities. Statistically significant differences were found between the water column and sediment trap bacteria. Relative abundances of Pelagibacter sp. and multiple members of the Flavobacteria, Actinobacteria, and α-Proteobacteria were elevated in water column samples, while trap samples contained members of the Roseobacter clade of α-Proteobacteria in high relative abundances. Our findings indicated that rapid changes – within 24 h of collection – occurred to the microbial community associated with aggregates from either bloom type. There was a little change in the bacterial assemblage after the initial 24-h incubation period. The most abundant early colonizer was a Sulfitobacter sp. This study provides further evidence that Roseobacters are rapid colonizers of marine aggregates and that colonization can occur on short timescales. This study further demonstrates that particle origin may be insignificant regarding the heterotrophic bacterial population that degrades them.

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