Copper induction of enhanced green fluorescent protein expression in Pleurotus ostreatus driven by laccase poxa1b promoter

Authors

  • Antonella Amore,

    1. Department of Chemical Sciences, University of Naples ‘Federico II’, Complesso Universitario Monte S. Angelo, Napoli, Italy
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  • Yoichi Honda,

    1. Graduate School of Agriculture, Kyoto University, Kyoto, Japan
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  • Vincenza Faraco

    Corresponding author
    1. School of Biotechnological Sciences, University of Naples ‘Federico II’, Napoli, Italy
    • Department of Chemical Sciences, University of Naples ‘Federico II’, Complesso Universitario Monte S. Angelo, Napoli, Italy
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Correspondence: Vincenza Faraco, Department of Chemical Sciences, University of Naples ‘Federico II’, Complesso Universitario Monte S. Angelo, via Cintia, 4 80126, Napoli, Italy. Tel.: +39 081 674315; fax: +39 081 674313; e-mail: vfaraco@unina.it

Abstract

In silico analyses of several laccase promoter sequences have shown the presence of many different responsive elements differentially distributed along the promoter sequences. Analysis of Pleurotus ostreatus laccase promoter poxa1b extending around 1400-bp upstream of the start codon showed the presence of several putative response elements, such as 10 metal-responsive elements. Development of a system for in vivo analysis of P. ostreatus laccase promoter poxa1b by enhanced green fluorescent protein expression was carried out, based on a polyethylene glycol–mediated procedure for fungal transformation. Quantitative measurement of fluorescence expressed in P. ostreatus transformants grown in the presence and in the absence of copper sulfate was performed, demonstrating an increase in expression level induced by the metal.

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