Taguchi optimization of duplex PCR for simultaneous identification of Staphylococcus aureus and Clostridium perfringens alpha toxins
Article first published online: 31 JAN 2013
© 2012 Federation of European Microbiological Societies Published by Blackwell Publishing Ltd. All rights reserved
FEMS Microbiology Letters
Volume 340, Issue 2, pages 93–100, March 2013
How to Cite
Ramakrishna, U. S., Kingston, J. J., Harishchandra Sripathi, M. and Batra, H. V. (2013), Taguchi optimization of duplex PCR for simultaneous identification of Staphylococcus aureus and Clostridium perfringens alpha toxins. FEMS Microbiology Letters, 340: 93–100. doi: 10.1111/1574-6968.12070
- Issue published online: 22 FEB 2013
- Article first published online: 31 JAN 2013
- Accepted manuscript online: 24 DEC 2012 12:23AM EST
- Manuscript Accepted: 18 DEC 2012
- Manuscript Revised: 12 NOV 2012
- Manuscript Received: 28 AUG 2012
- Junior Research Fellowship of Council for Scientific and Industrial Research, Government of India
|fml12070-sup-0001-SupplementaryData1.pdf||application/PDF||43K||Data S1. ClustalW Multiple Sequence Alignment of hla gene sequences retrieved from NCBI-Genbank database showing conserved regions of primers used in the study.|
Data S2. Orthogonal arrays for 4 critical variables A, B, C & D each at three levels a, b & c.
Data S3. Electropherograms of PCR amplicons generated in 9 combinations of duplex PCR mixes described in Data s2.
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