Identification of human plasma proteins associated with the cell wall of the pathogenic fungus Paracoccidioides brasiliensis

Authors

  • Larissa V.G. Longo,

    1. Escola Paulista de Medicina, Universidade Federal de São Paulo, UNIFESP, SP, Brazil
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  • Ernesto S. Nakayasu,

    1. Border Biomedical Research Center, Department of Biological Sciences, University of Texas at El Paso (UTEP), El Paso, TX, USA
    Current affiliation:
    1. Biological Science Division, Pacific Northwest National Laboratory, Richland, WA, USA
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  • Alisson L. Matsuo,

    1. Escola Paulista de Medicina, Universidade Federal de São Paulo, UNIFESP, SP, Brazil
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  • Roberta Peres da Silva,

    1. Escola Paulista de Medicina, Universidade Federal de São Paulo, UNIFESP, SP, Brazil
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  • Tiago J.P. Sobreira,

    1. National Laboratory for Biosciences (LNBio), National Center for Research in Energy and Materials, Campinas, SP, Brazil
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  • Milene C. Vallejo,

    1. Escola Paulista de Medicina, Universidade Federal de São Paulo, UNIFESP, SP, Brazil
    Current affiliation:
    1. Division of Nephrology & Hypertension and Department of Cell & Developmental Biology, Oregon Health & Science University, Portland, OR, USA
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  • Luciane Ganiko,

    1. Border Biomedical Research Center, Department of Biological Sciences, University of Texas at El Paso (UTEP), El Paso, TX, USA
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  • Igor C. Almeida,

    Corresponding author
    • Border Biomedical Research Center, Department of Biological Sciences, University of Texas at El Paso (UTEP), El Paso, TX, USA
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  • Rosana Puccia

    Corresponding author
    • Escola Paulista de Medicina, Universidade Federal de São Paulo, UNIFESP, SP, Brazil
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Correspondence: Rosana Puccia, DMIP, UNIFESP, Rua Botucatu, 862, 8º andar, São Paulo, SP 04023-062, Brazil. Tel.: +55 11 55764551; fax: +55 11 5571 5877; e-mail: ropuccia@gmail.com

Abstract

Paracoccidioides brasiliensis and Paracoccidioides lutzii are thermodimorphic species that cause paracoccidioidomycosis. The cell wall is the outermost fungal organelle to form an interface with the host. A number of host effector compounds, including immunologically active molecules, circulate in the plasma. In the present work, we extracted cell-wall-associated proteins from the yeast pathogenic phase of P. brasiliensis, isolate Pb3, grown in the presence of human plasma and analyzed bound plasma proteins by liquid chromatography–tandem mass spectrometry. Transport, complement activation/regulation, and coagulation pathway were the most abundant functional groups identified. Proteins related to iron/copper acquisition, immunoglobulins, and protease inhibitors were also detected. Several human plasma proteins described here have not been previously reported as interacting with fungal components, specifically, clusterin, hemopexin, transthyretin, ceruloplasmin, alpha-1-antitrypsin, apolipoprotein A-I, and apolipoprotein B-100. Additionally, we observed increased phagocytosis by J774.16 macrophages of Pb3 grown in plasma, suggesting that plasma proteins interacting with P. brasiliensis cell wall might be interfering in the fungal relationship with the host.

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