Sensitive and substrate-specific detection of metabolically active microorganisms in natural microbial consortia using community isotope arrays
Article first published online: 22 MAR 2013
© 2013 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd. All rights reserved
FEMS Microbiology Letters
Volume 342, Issue 1, pages 70–75, May 2013
How to Cite
Tourlousse, D. M., Kurisu, F., Tobino, T. and Furumai, H. (2013), Sensitive and substrate-specific detection of metabolically active microorganisms in natural microbial consortia using community isotope arrays. FEMS Microbiology Letters, 342: 70–75. doi: 10.1111/1574-6968.12112
- Issue published online: 11 APR 2013
- Article first published online: 22 MAR 2013
- Accepted manuscript online: 26 FEB 2013 12:25PM EST
- Manuscript Revised: 20 FEB 2013
- Manuscript Accepted: 20 FEB 2013
- Manuscript Received: 30 DEC 2012
- Nippon Steel Corporation
- kakenhi Grant-in-Aid for Scientific Research. Grant Number: 23710086
Fig. S1. (a) Dynamics of 14C-phenol degradation and assimilation of 14C-atoms (radioactivity) into the microbial biomass.
Fig. S2. Normalized abundance of additional clones with positive signal on the CIArray in the density-resolved DNA fractions prepared from sample incubated with a total of 330 mg L−1 of 12C-phenol (shown in blue) and 13C-phenol (shown in red).
Fig. S3. Phylogenetic tree showing the affiliation of the unclassified gammaproteobacterial OTUs LE05 and LE12 (see Table S4).
Table S1. Reactor influent composition, operational parameters and dominant phenol-degrading bacteria as determined by Sueoka et al. (2009) and in this study.
Table S2. Best Blast Hits (BBH) and taxonomic assignment of the partial fosmid clone sequences.
Table S3. Fosmid-clone-specific primers.
Table S4 Summary of the 16S rRNA gene clone library data (partial sequences of 700 bp were generated using the 27F primer).
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