fml12123-sup-0001-FigS1-S6_TableS1.docxWord document4310K

Fig. S1. Gram staining (positive) of Exiguobacterium MPO cells viewed at 40× magnification.

Fig. S2. Cross-feeding bioassay of A. tumefaceins A136 bioreporter responding to MPO culture by producing a blue colouration due to X-gal degradation, indicative of the presence of AHL.

Fig. S3. Results for inhibition plate assay with CV026 bioreporter: Positive control A-10 μM OOHL,B-100 μM OOHL,C-Octanoyl homoserine lactone(just to check activation of CV026), Negative control D-methanol, E-MPO extract in methanol.

Fig. S4. LCMS data for MPO indicating OOHL with the presence of the characteristic lactone fragment at m/z 102 and the molecular ion peak (M+H)+ 242.

Fig. S5. EC50 for the MPO extract luminescence-inducing activity in the E.coli JM109 (psb1075) reporter.

Fig. S6. 30% and 16% reduction in EPS in OOHL and extract supplemented cultures of MPO with respect to control is observed respectively.(P value = 0.04).

Table S1. List of primers used in sequencing.

Table S2. List of the primers used in the qPCR study.

Data S1. Details of the parameter used in the in silico analysis of ExgR and ExgI.

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