Production of acylated homoserine lactone by Gram-positive bacteria isolated from marine water
Article first published online: 2 APR 2013
© 2013 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd. All rights reserved
FEMS Microbiology Letters
Volume 343, Issue 1, pages 34–41, June 2013
How to Cite
Biswa, P. and Doble, M. (2013), Production of acylated homoserine lactone by Gram-positive bacteria isolated from marine water. FEMS Microbiology Letters, 343: 34–41. doi: 10.1111/1574-6968.12123
- Issue published online: 8 MAY 2013
- Article first published online: 2 APR 2013
- Accepted manuscript online: 13 MAR 2013 07:56AM EST
- Manuscript Accepted: 4 MAR 2013
- Manuscript Revised: 1 MAR 2013
- Manuscript Received: 16 JAN 2013
- Council of Scientific and Industrial Research
Fig. S1. Gram staining (positive) of Exiguobacterium MPO cells viewed at 40× magnification.
Fig. S2. Cross-feeding bioassay of A. tumefaceins A136 bioreporter responding to MPO culture by producing a blue colouration due to X-gal degradation, indicative of the presence of AHL.
Fig. S3. Results for inhibition plate assay with CV026 bioreporter: Positive control A-10 μM OOHL,B-100 μM OOHL,C-Octanoyl homoserine lactone(just to check activation of CV026), Negative control D-methanol, E-MPO extract in methanol.
Fig. S4. LCMS data for MPO indicating OOHL with the presence of the characteristic lactone fragment at m/z 102 and the molecular ion peak (M+H)+ 242.
Fig. S5. EC50 for the MPO extract luminescence-inducing activity in the E.coli JM109 (psb1075) reporter.
Fig. S6. 30% and 16% reduction in EPS in OOHL and extract supplemented cultures of MPO with respect to control is observed respectively.(P value = 0.04).
Table S1. List of primers used in sequencing.
Table S2. List of the primers used in the qPCR study.
Data S1. Details of the parameter used in the in silico analysis of ExgR and ExgI.
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