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Fig. S1. SDS-PAGE (a) and molecular mass determination (b) of S. degradans MalQ. Lane 1: molecular mass marker; lane 2: cell-free extracts of pET29b without malQ; lane 3: cell-free extracts of pET29b with malQ; lane 4: purified MalQ. Molecular mass standards: ferritin (440 kDa), β-amylase (200 kDa), amyloglucosidase (97 kDa), and albumin (68 kDa).

Fig. S2. Identification of the linearized reaction products by MalQ. The reaction mixture (50 μL) containing 0.2% (w/v) maltotriose in 25 mM Tris–HCl (pH 8.5) and MalQ (1 μg) was incubated at 35 °C for 4 h. S, standard (from glucose to maltoheptaose [10 mM]); Lane 1, absence of MalQ; Lane 2, presence of MalQ; Lane 3, glucoamylase treatment (16 U, incubated at 50 °C for 8 h) after the MalQ enzyme reaction.

Table S1. Bacterial strains, plasmids, and oligonucleotides used in this study.

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