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fml12178-sup-0001-FigS1.tifimage/tif8942KFig. S1. Yeast two-hybrid screen to study the interaction of the O-demethylase components. For the designation of recombinant yeast strains (1-12, A-L), see Table S3.
fml12178-sup-0002-FigS2.tifimage/tif1555KFig. S2. Positive interactions of the O-demethylase components and its fragments obtained by yeast two-hybrid screen (see also Fig. S1). The yeast cells were applied in decreasing concentrations from left to right. The dilution factors are indicated on top of each plate. 2B: AE × AE, 2C/3B: AE × AE1-133, 3C: AE1-133 × AE1-133, 3D: AE1-133 × AE134-598, 6I: CP × MT I. For further designation of the recombinant yeast strains, see Table S3.
fml12178-sup-0003-TableS1.docxWord document13KTable S1. Oligonucleotides used for the cloning of the genes encoding the corrinoid proteins of the syringate- (CPsyr) and guaiacol-O-demethylase (CPgua). For details, see ‘Materials and Methods’ section.
fml12178-sup-0004-TableS2.docxWord document15KTable S2. Oligonucleotides used for the plasmid construction for yeast two-hybrid assays. For details, see ‘Materials and Methods’ section.
fml12178-sup-0005-TableS3.docxWord document13KTable S3. Designation of the plasmids used in the yeast two-hybrid screen. AE = activating enzyme, CP = corrinoid protein, MT = methyltransferase.

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