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fml12183-sup-0001-FigS1.tifimage/tif4236KFig. S1. Schematic maps of plasmids and their restriction sites.
fml12183-sup-0002-FigS2.tifimage/tif1011KFig. S2. Determination of the cloned avermectin biosynthetic gene cluster in linear plasmid pZR695-p by Southern hybridization.
fml12183-sup-0003-FigS3.tifimage/tif1761KFig. S3. Schematic of the recombinational step for deletion of a SCP1 segment.
fml12183-sup-0004-FigS4.tifimage/tif2749K;Fig. S4. Schematic of the recombinational insertion steps for adding large DNA segments from overlapping cosmids.
fml12183-sup-0005-FigS5.tifimage/tif1420KFig. S5. Locations of a PCR segment (‘A’) and four overlapping cosmid inserts (spn-1–4) in the spinosad biosynthetic gene cluster.
fml12183-sup-0006-FigS6.tifimage/tif821KFig. S6. Detection of SCP1 and its derived linear plasmids in the recombinational experiments.
fml12183-sup-0007-FigS7.tifimage/tif2122KFig. S7. Determination of the cloned spinosad biosynthetic gene cluster in linear plasmid pZR669-p by digestion with EcoRV and XhoI and Southern hybridization with mixed probes (1-kb ladder, four cosmids spn-1–4).
fml12183-sup-0008-TableS1.docWord document35KTable S1. The primers used for cloning the avermectin biosynthetic gene cluster into SCP1.

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