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Table S1. Bacterial strains and plasmids used in this study.

Table S2. Primers and oligonucleotides used in this study. Sites used in cloning, or, in the case of EMSAs, the location of the motif, are underlined.

Data S1. Stoichiometry of the His6-BkaRMtb-DNA complex.

Data S2. RT-PCR results for the run of genes fadE19-bkdA performed with cDNA derived from ΔbkaRMsm.

Data S3. EMSA with purified His6-BkaR and probes containing the motifs predicted by MAST in M. smegmatis and M. tuberculosis.

Data S4. Overview of branched chain amino acid metabolism and the possible involvement of the bkaR regulon.

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