bkaR is a TetR-type repressor that controls an operon associated with branched-chain keto-acid metabolism in Mycobacteria
Article first published online: 8 JUL 2013
© 2013 The Authors. FEMS Microbiology Letters published by John Wiley & Sons Ltd on behalf of the Federation of European Microbiological Societies.
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FEMS Microbiology Letters
Volume 345, Issue 2, pages 132–140, August 2013
How to Cite
Balhana, R. J.C., Swanston, S. N., Coade, S., Withers, M., Sikder, M. H., Stoker, N. G. and Kendall, S. L. (2013), bkaR is a TetR-type repressor that controls an operon associated with branched-chain keto-acid metabolism in Mycobacteria. FEMS Microbiology Letters, 345: 132–140. doi: 10.1111/1574-6968.12196
- Issue published online: 19 JUL 2013
- Article first published online: 8 JUL 2013
- Accepted manuscript online: 13 JUN 2013 09:51AM EST
- Manuscript Revised: 6 JUN 2013
- Manuscript Accepted: 6 JUN 2013
- Manuscript Received: 23 MAY 2013
- Wellcome trust. Grant Number: 073237
- MRC. Grant Number: U117581288
Table S1. Bacterial strains and plasmids used in this study.
Table S2. Primers and oligonucleotides used in this study. Sites used in cloning, or, in the case of EMSAs, the location of the motif, are underlined.
Data S1. Stoichiometry of the His6-BkaRMtb-DNA complex.
Data S2. RT-PCR results for the run of genes fadE19-bkdA performed with cDNA derived from ΔbkaRMsm.
Data S3. EMSA with purified His6-BkaR and probes containing the motifs predicted by MAST in M. smegmatis and M. tuberculosis.
Data S4. Overview of branched chain amino acid metabolism and the possible involvement of the bkaR regulon.
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