An attenuated mutant of the Rv1747 ATP-binding cassette transporter of Mycobacterium tuberculosis and a mutant of its cognate kinase, PknF, show increased expression of the efflux pump-related iniBAC operon
Version of Record online: 23 AUG 2013
© 2013 The Authors. FEMS Microbiology Letters published by John Wiley & Sons Ltd on behalf of the Federation of European Microbiological Societies.
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FEMS Microbiology Letters
Volume 347, Issue 2, pages 107–115, October 2013
How to Cite
Spivey, V. L., Whalan, R. H., Hirst, E. M. A., Smerdon, S. J. and Buxton, R. S. (2013), An attenuated mutant of the Rv1747 ATP-binding cassette transporter of Mycobacterium tuberculosis and a mutant of its cognate kinase, PknF, show increased expression of the efflux pump-related iniBAC operon. FEMS Microbiology Letters, 347: 107–115. doi: 10.1111/1574-6968.12230
- Issue online: 24 SEP 2013
- Version of Record online: 23 AUG 2013
- Accepted manuscript online: 5 AUG 2013 06:56AM EST
- Manuscript Revised: 24 JUL 2013
- Manuscript Accepted: 24 JUL 2013
- Manuscript Received: 19 JUN 2013
- MRC studentship
- Medical Research Council. Grant Numbers: U117585867, U117584228
|fml12230-sup-0001-FigS1.tif||image/tif||1484K||Fig. S1. Growth inhibition assays assessing the susceptibility of WT H37Rv, ΔpknF and ΔRv1747 strains to a range of drug and stress reagents.|
|fml12230-sup-0002-FigS2.tif||image/tif||8443K||Fig. S2. β-Galactosidase assays on the pknF promoter-lacZ strain and pEJ414 control strain in Mycobacterium tuberculosis after a panel of treatments.|
|fml12230-sup-0003-FigS3.tif||image/tif||25513K||Fig. S3. Transmission electron micrographs of Mycobacterium tuberculosis comparing cell wall structure in (a) WT H37Rv, ΔRv1747 and Rv1747 complement strains, and (b) WT H37Rv, ΔpknF and pknF complement strains.|
|fml12230-sup-0004-FigS4.tif||image/tif||6342K||Fig. S4. Mycobacterium tuberculosis whole cell ELISAs comparing the levels of ManLAM in H37Rv WT, ΔRv1747 and Rv1747 complement strains.|
|fml12230-sup-0005-MaterialsandMethods.docx||Word document||20K||Appendix S1. Supplementary Materials and methods|
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