Fig. S1. Organization of dnd genes clusters in 17 members of the phylum Bacteroidetes.

Fig. S2. Alignment of eleven DndEi homologues detected in members of the phylum Bacteroidetes.

Fig. S3. (A) Inferred phylogenetic relationship of 15 members of the phylum Bacteroidetes carrying a dnd cluster (or their close relatives) on the basis of 16S rRNA gene sequences. Included in the phylogeny are 16S sequences from : Flavobacterium psychrophilum NCIMB 1947T (HM443879.1), Flavobacterium indicum CIP 109464T (NR_074422.1), Kordia algicida OT-1 (NR_027568.1), Flavobacteriaceae bacterium 3519-10 (EU694411.1), Riemerella anatipestifer DSM 15868 (NR_074429.1), Belliella baltica DSM 15883 (NR_025599.1), Microscilla marina NBRC 16560 (AB681071.1), Haliscomenobacter hydrossis DSM 1100 (NR_074420.1), Parabacteroides goldsteinii JCM13446T (EU136697.1), Bacteroides faecis MAJ27 (GQ496624.1), Bacteroides finegoldii DSM 17565 (NR_041313.1), Bacteroides xylanisolvens XB1A (NR_042499.1), Paraprevotella xylaniphila YIT 11841 (NR_041627.1), Prevotella amnii CCUG 53648T (NR_042587.1), Prevotella bivia ATCC 29303 (NR_044629.1). 16S sequences from Bacteroides sp. 2_1_33B and Cyclobacteriaceae bacterium AK24 were not included in this analysis. (B) Phylogenetic analysis performed on concatened DndC and DndD proteins sequences, found in all the Bacteroidetes dnd clusters reported. * indicate dnd gene clusters containing a DndEi homolog. Accessions numbers are shown in Table 1. Bootstrap values (100 replicates) are shown at the nodes and scales represent inferred evolutionary distance.

Table S1. primer sequences used in the RT-PCR analysis of the Flavobacterium indicum dnd locus.

Table S2. Dnd proteins homologs of Flavobacterium strains identified.

Please note: Wiley Blackwell is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.