Raman spectrometric discrimination of flexirubin pigments from two genera of Bacteroidetes

Authors

  • Jan Jehlička,

    Corresponding author
    1. Institute of Geochemistry, Mineralogy and Mineral Resources, Charles University, Prague, Czech Republic
    • Correspondence: Jan Jehlička, Institute of Geochemistry, Mineralogy and Mineral Resources, Charles University, Albertov 6, 12843 Prague, Czech Republic.

      Tel.: +420 221 95 1503;

      fax: +420 221 95 1429;

      e-mail: jehlicka@natur.cuni.cz

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  • Kateřina Osterrothová,

    1. Institute of Geochemistry, Mineralogy and Mineral Resources, Charles University, Prague, Czech Republic
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  • Aharon Oren,

    1. Department of Plant and Environmental Sciences, The Institute of Life Sciences, The Hebrew University of Jerusalem, Jerusalem, Israel
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  • Howell G. M. Edwards

    1. Centre for Astrobiology and Extremophiles Research, School of Life Sciences, University of Bradford, Bradford, UK
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Abstract

Flexirubins are specific polyene pigments produced by several genera of Bacteroidetes. Colonies and cell extracts of Flavobacterium johnsoniae and Flexibacter elegans have been investigated by Raman spectroscopy to show that this fast and non-destructive technique can be used to differentiate these pigments from carotenoids and to compare the flexirubin content of the two microorganisms. The presence or absence of certain distinguishing features in the CH combination band region at 2500–2750 cm−1 can assist in the discrimination between the two flexirubins investigated. Raman spectroscopy is thus a suitable tool not only to detect flexirubin pigments in bacterial cells, but also to further characterize the pigments present in members of the Bacteroidetes genera that are rich in flexirubins.

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