Transcriptional downregulation of agr expression in Staphylococcus aureus during growth in human serum can be overcome by constitutively active mutant forms of the sensor kinase AgrC
Version of Record online: 18 NOV 2013
© 2013 The Authors. FEMS Microbiology Letters Published by John Wiley & Sons Ltd on behalf of the Federation of European Microbiological Societies.
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FEMS Microbiology Letters
Volume 349, Issue 2, pages 153–162, December 2013
How to Cite
James, E. H., Edwards, A. M. and Wigneshweraraj, S. (2013), Transcriptional downregulation of agr expression in Staphylococcus aureus during growth in human serum can be overcome by constitutively active mutant forms of the sensor kinase AgrC. FEMS Microbiology Letters, 349: 153–162. doi: 10.1111/1574-6968.12309
- Issue online: 21 NOV 2013
- Version of Record online: 18 NOV 2013
- Accepted manuscript online: 24 OCT 2013 11:31AM EST
- Manuscript Accepted: 17 OCT 2013
- Manuscript Revised: 11 OCT 2013
- Manuscript Received: 10 SEP 2013
Fig. S1. Photograph of a bacterial growth plate (one of three biological replicates) taken on blue light transluminator to determine the stability of transcriptional reporters used in this study.
Fig S2. Western blot showing specificity of anti-AgrA polycolonal antibody to AgrA.
Table S1. Primers used for cloning, colony PCR, qRT-PCR and site-directed mutagenesis (SDM).
Data S1. Materials and methods.
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