Informatic prediction of Cheddar cheese flavor pathway changes due to sodium substitution
Version of Record online: 9 DEC 2013
© 2013 Federation of European Microbiological Societies. Published by John Wiley & Sons Ltd. All rights reserved
FEMS Microbiology Letters
Volume 350, Issue 2, pages 231–238, January 2014
How to Cite
Ganesan, B. and Brown, K. (2014), Informatic prediction of Cheddar cheese flavor pathway changes due to sodium substitution. FEMS Microbiology Letters, 350: 231–238. doi: 10.1111/1574-6968.12328
- Issue online: 27 JAN 2014
- Version of Record online: 9 DEC 2013
- Accepted manuscript online: 19 NOV 2013 05:35AM EST
- Manuscript Accepted: 20 OCT 2013
- Manuscript Received: 23 SEP 2013
- Dairy Management Inc.
- lactic acid bacteria;
Increased interest in reduced and low sodium dairy foods generates flavor issues for cheeses. Sodium is partly replaced with potassium or calcium to sustain the salty flavor perception, but the other cations may also alter metabolic routes and the resulting flavor development in aged cheeses. The effect of some cations on selected metabolic enzyme activity and on lactic acid bacterial physiology and enzymology has been documented. Potassium, for example, is an activator of 40 enzymes and inhibits 25 enzymes. Currently, we can visualize the effects of these cations only as lists inside metabolic databases such as MetaCyc. By visualizing the impact of these activating and inhibitory activities as biochemical pathways inside a metabolic database, we can understand their relevance, predict, and eventually dictate the aging process of cheeses with cations that replace sodium. As examples, we reconstructed new metabolic databases that illustrate the effect of potassium on flavor-related enzymes as microbial pathways. After metabolic reconstruction and analysis, we found that 153 pathways of lactic acid bacteria are affected due to enzymes likely to be activated or inactivated by potassium. These pathways are primarily linked to sugar metabolism, acid production, and amino acid biosynthesis and degradation that relate to Cheddar cheese flavor.