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Fig. S1. Flow chart of experimental procedures.

Fig. S2. EcoRI-HindIII digests of representative ApR plasmids recovered after transformation of total genomic DNA from ApR GmR PstQ derivatives into Escherichia coli JM109.

Fig. S3. PCR spanning the region between oriV and bla in plasmids containing deletions in ISPst4 (pUS51-57), the parental plasmid pUS23 (no ISPst4), the original ISPst4-containing plasmid (p13A), and the derivative of p13A with the reporter gene region deleted (pUS50).

Fig. S4. Representative LB-Ap agar plates showing results of transformation of 1 µg of different p13A plasmid deletion derivatives into PstQ.

Table S1. Bacterial strains, plasmids and oligonucleotides.

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