Identification of novel sRNAs in Brucella abortus 2308

Authors

  • Hao Dong,

    1. Key Laboratory of Animal Epidemiology and Zoonosis of Ministry of Agriculture, College of Veterinary Medicine, China Agricultural University, Beijing, China
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  • Xiaowei Peng,

    1. Key Laboratory of Animal Epidemiology and Zoonosis of Ministry of Agriculture, College of Veterinary Medicine, China Agricultural University, Beijing, China
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  • Ning Wang,

    1. Key Laboratory of Animal Epidemiology and Zoonosis of Ministry of Agriculture, College of Veterinary Medicine, China Agricultural University, Beijing, China
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  • Qingmin Wu

    Corresponding author
    1. Key Laboratory of Animal Epidemiology and Zoonosis of Ministry of Agriculture, College of Veterinary Medicine, China Agricultural University, Beijing, China
    • Correspondence: Qingmin Wu, Key Laboratory of Animal Epidemiology and Zoonosis of Ministry of Agriculture, College of Veterinary Medicine, China Agricultural University, Beijing 100193, China. Tel.: +86 10 6273 3901; fax: +86 10 6273 3901;

      e-mail: wuqm@cau.edu.cn

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Abstract

Involved in diverse biological processes, bacterial sRNAs are novel regulators of gene expression involved in a wide array of biological processes. To identify sRNAs in Brucella abortus, we performed a genome-wide computational prediction with integrated sipht and napp results. In total, 129 sRNA candidates were identified, of which 112 were novel sRNA. Twenty novel sRNA candidates were tested by RT-PCR and seven could be verified. The putative targets of these sRNAs were also predicted and verified. This study provides a significant resource for the future study of sRNAs, as well as how sRNAs influence B. abortus physiology and pathogenesis.

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