Interplay among Pseudomonas syringae HrpR, HrpS and HrpV proteins for regulation of the type III secretion system
Article first published online: 19 JUN 2014
© 2014 The Authors. FEMS Microbiology Letters published by John Wiley & Sons Ltd on behalf of Federation of European Microbiological Societies.
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FEMS Microbiology Letters
Special Issue: Pseudomonas
Volume 356, Issue 2, pages 201–211, July 2014
How to Cite
Jovanovic, M., Lawton, E., Schumacher, J. and Buck, M. (2014), Interplay among Pseudomonas syringae HrpR, HrpS and HrpV proteins for regulation of the type III secretion system. FEMS Microbiology Letters, 356: 201–211. doi: 10.1111/1574-6968.12476
- Issue published online: 24 JUL 2014
- Article first published online: 19 JUN 2014
- Accepted manuscript online: 26 MAY 2014 04:09AM EST
- Manuscript Accepted: 16 MAY 2014
- Manuscript Revised: 15 MAY 2014
- Manuscript Received: 7 FEB 2014
- Leverhulme Trust. Grant Number: F/07 058/BM
Fig. S1. The amino acid substitutions HrpS(I26N)/(R90H)/(R185H)/(H251Q) on the structural model of HrpS, based on the AAA+ domain of PspF.
Fig. S2. Transcription activity in vivo of HrpS variants, within HrpRS complexes, that escapes negative regulation by HrpV.
Fig. S3. Binding interactions between the HrpS variants I26, R90, R185 and H251 fused to T18 AC fragment and assayed in the presence of: (a) HrpV fused to T25 AC fragment, (b) HrpR fused to T25 AC fragment, (c) HrpS fused to T25 AC fragment.
Fig. S4. Western blotting of the Hrp proteins expressed in the BACTH system recognized by corresponding antibody.
Table S1. Bacterial strains and plasmids used in this study.
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