A magnetosome-associated cytochrome MamP is critical for magnetite crystal growth during the exponential growth phase
Version of Record online: 4 AUG 2014
© 2014 Federation of European Microbiological Societies. Published by John Wiley & Sons Ltd. All rights reserved
FEMS Microbiology Letters
Volume 358, Issue 1, pages 21–29, September 2014
How to Cite
Taoka, A., Eguchi, Y., Mise, S., Oestreicher, Z., Uno, F. and Fukumori, Y. (2014), A magnetosome-associated cytochrome MamP is critical for magnetite crystal growth during the exponential growth phase. FEMS Microbiology Letters, 358: 21–29. doi: 10.1111/1574-6968.12541
- Issue online: 4 SEP 2014
- Version of Record online: 4 AUG 2014
- Accepted manuscript online: 21 JUL 2014 09:45AM EST
- Manuscript Accepted: 11 JUL 2014
- Manuscript Revised: 4 JUL 2014
- Manuscript Received: 26 APR 2014
- MEXT KAKENHI. Grant Number: 24117007
- JSPS KAKENHI. Grant Number: 25850051
- Institute for Fermentation, Osaka (IFO)
|fml12541-sup-0001-FigS1.eps||image/eps||1225K||Fig. S1. Primary sequence of MamP.|
|fml12541-sup-0002-FigS2.eps||image/eps||1656K||Fig. S2. SDS-PAGE gel profiles of the proteins extracted from the soluble fraction (lane 1) and membrane fractions (lane 2) prepared from Escherichia coli C41(DE3) (pET29b-mamP).|
|fml12541-sup-0003-FigS3.eps||image/eps||909K||Fig. S3. Growth curve (open circles) and magnetism (Cmag) (square) of Magnetospirillum magneticum AMB-1 under the same culture conditions for this study.|
|fml12541-sup-0004-FigS4.eps||image/eps||2465K||Fig. S4. Histogram for magnetite crystal size in cells from wild-type (black bar) and MamP overproduced cells (white bar) that were cultivated for 24 h; exponential growth phase, (a), 72 h; stationary growth phase, (b) and 144 h; stationary growth phase, (c).|
Table S1. Bacterial stains and plasmids used in this study.
Table S2. Primers used in this study.
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