S-layers: principles and applications

Authors

  • Uwe B. Sleytr,

    Corresponding author
    1. Institute of Biophysics, Department of Nanobiotechnology, University of Natural Resources and Life Sciences, Vienna, Austria
    • Correspondence: Uwe B. Sleytr, Institute of Biophysics, Department of Nanobiotechnology, University of Natural Resources and Life Sciences, Muthgasse 11, 1190 Vienna, Austria. Tel.: +43 1 47654 2201; fax: +43 1 4789112; e-mail: uwe.sleytr@boku.ac.at

    Search for more papers by this author
  • Bernhard Schuster,

    1. Institute of Synthetic Biology, Department of Nanobiotechnology, University of Natural Resources and Life Sciences, Vienna, Austria
    Search for more papers by this author
  • Eva-Maria Egelseer,

    1. Institute of Biophysics, Department of Nanobiotechnology, University of Natural Resources and Life Sciences, Vienna, Austria
    Search for more papers by this author
  • Dietmar Pum

    1. Institute of Biophysics, Department of Nanobiotechnology, University of Natural Resources and Life Sciences, Vienna, Austria
    Search for more papers by this author

  • [The copyright line for this article was changed on 7 October 2014 after original online publication]

Abstract

Monomolecular arrays of protein or glycoprotein subunits forming surface layers (S-layers) are one of the most commonly observed prokaryotic cell envelope components. S-layers are generally the most abundantly expressed proteins, have been observed in species of nearly every taxonomical group of walled bacteria, and represent an almost universal feature of archaeal envelopes. The isoporous lattices completely covering the cell surface provide organisms with various selection advantages including functioning as protective coats, molecular sieves and ion traps, as structures involved in surface recognition and cell adhesion, and as antifouling layers. S-layers are also identified to contribute to virulence when present as a structural component of pathogens. In Archaea, most of which possess S-layers as exclusive wall component, they are involved in determining cell shape and cell division. Studies on structure, chemistry, genetics, assembly, function, and evolutionary relationship of S-layers revealed considerable application potential in (nano)biotechnology, biomimetics, biomedicine, and synthetic biology.

Ancillary