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Authentication of Pure L-Leucine Products Manufactured in China by Discriminating between Plant and Animal Sources Using Nitrogen Stable Isotope Technique

Authors

  • Jingyu Huang,

    1. Ministry of Education Key Laboratory of Integrated Regulation and Resource Development on Shallow Lakes, Hohai Univ., China
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  • Philip N. Nkrumah,

    Corresponding author
    1. Dept. of Agricultural Engineering, Kwame Nkrumah Univ. of Science and Technology, PMB, Kumasi, Ghana
    • Ministry of Education Key Laboratory of Integrated Regulation and Resource Development on Shallow Lakes, Hohai Univ., China
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  • Gloria Appiah-Sefah,

    1. Ministry of Education Key Laboratory of Integrated Regulation and Resource Development on Shallow Lakes, Hohai Univ., China
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  • Shijiang Tang

    1. Chengdu Keyuan Biotechnology Co. Ltd., Sichuan, China
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Direct inquiries to author Nkrumah (E-mail: philiponti1209@yahoo.com)

Abstract

 L-leucine products among other branched chain amino acid supplements are highly susceptible to economically motivated adulteration. Curbing this menace is critical and timely. Hence, the δ15N composition of the L-leucine derived from plants and animals sources was estimated. The trophic enrichment phenomenon of δ15N composition was utilized to elucidate the sources. We finally established the distinction between the respective sources.

Samples of plant sources (maize and soybean) and that of animal sources (pig fur and duck feather) were analyzed for δ15N isotopic signatures. An elemental analyzer which was connected to an isotope ratio mass spectrometer operated in the continuous flow mode was utilized. The raw materials were obtained from China. Statistical analysis was performed using descriptive statistics and one-way analysis of variance.

The results indicated lower δ15N values of range –0.7344‰ to 2.384‰ and 1.032‰ to 2.064‰ for maize and soybean samples, respectively. Whereas, a range of 3.860‰ to 6.011‰ and 5.875‰ to 6.011‰ was, respectively, detected in pig fur and duck feather samples. The δ15N difference in plants and animals samples was significant (F = 165.0; P = 1.675 E-10 for maize and pig fur samples; F = 212.8; P = 0.0001284 for soybean and duck feather samples).

It was observed that δ15N trophic enrichment is helpful in elucidating the respective sources. The authors can emphatically assert that the range of δ15N composition of L-leucine derived from plants sources within the study area is –1.000‰ to 3.000‰ whereas the range in animal sources is 4.000‰ to 9.000‰.

Practical Application This study provides a reliable approach in verifying the authenticity of not only L-leucine products but also other branched chain amino acid supplements and thereby would help in fraud detection of any economically motivated adulteration and mislabeling of these products. When coupled with H and O stable isotope techniques, the region-of-origin of the detected adulteration can also be traced successfully. It therefore serves as a guide to food regulatory bodies, food scientists, retailers of these products, consumers, and the general public at large.

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