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Figure S1. TLC analysis of purified P. decumbens naringinase incubated with rutin at pH 6.0 and 37 °C. Lane S, standard maltooligosaccharides (G1–G7); lane 1, α-L-rhamnose; lane 2, rutin; lane 3, Aspergillus sojae naringinase incubated with rutin for 12 h; lane 4, P. decumbens naringinase incubated with rutin for 12 h; lane 5, Q-3-G; lane 6, quercetin. Naringinase (0.04 mg/mL) and rutin (1 mM) were incubated together in 50 mM sodium citrate buffer (pH 6.0) containing 20% DMSO.

Figure S2. HPLC analysis of purified P. decumbens naringinase incubated with rutin at pH 6.0 and 37 °C. (A) rutin (B) P. decumbens naringinase incubated with rutin for 3 h (C) P. decumbens naringinase incubated with rutin for 12 h. AU means an absorbance at 254 nm. 1, rutin; 2, Q-3-G; 3, quercetin.

Figure S3. The comparison of turbidity of rutin (A), Q-3-G (B), and quercetin (C) in deionized and distilled water. Each compound was finally suspended at 1 mM.

Figure S4. Time-course analysis of purified P. decumbens naringinase incubated with rutin (A) and repetitive batch-type production of Q-3-G (B). Naringinase (0.04 mg/mL) was incubated in 50 mM sodium citrate buffer (pH 6.0) containing 20% DMSO (A) or 30% ethanol (B) for 12 h at 37 °C. The reaction mixture was analyzed by HPLC. Rutin, ▪; Q-3-G, ●; quercetin, ∆.

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