M: Food Microbiology & Safety
Development and Application of a Method for the Analysis of 9 Mycotoxins in Maize by HPLC-MS/MS
Article first published online: 23 SEP 2013
© 2013 Institute of Food Technologists®
Journal of Food Science
Volume 78, Issue 11, pages M1752–M1756, November 2013
How to Cite
Wang, Y., Xiao, C., Guo, J., Yuan, Y., Wang, J., Liu, L. and Yue, T. (2013), Development and Application of a Method for the Analysis of 9 Mycotoxins in Maize by HPLC-MS/MS. Journal of Food Science, 78: M1752–M1756. doi: 10.1111/1750-3841.12187
- Issue published online: 19 NOV 2013
- Article first published online: 23 SEP 2013
- Manuscript Accepted: 9 MAY 2013
- Manuscript Received: 30 DEC 2012
- Basic Science Research Foundation. Grant Number: Z109021104
- OASIS HLB;
A reliable and sensitive liquid chromatography/tandem mass spectrometry (LC-MS/MS) method was developed for the simultaneous determination of aflatoxins (AFB1, AFB2, AFG1, and AFG2), ochratoxin A (OTA), deoxynivalenol (DON), zearalenone (ZEA), fumonisin B1 (FB1), and T2-toxin in maize. The samples were first extracted using acetonitrile: water: acetic acid (79 : 20 : 1), and then further cleaned-up using OASIS HLB cartridge. Optimum conditions for the extraction and chromatographic separation were investigated. The mean recoveries of mycotoxins in spiked maize ranged from 68.3% to 94.3%. Limits of detection and quantification ranged from 0.01 to 0.64 μg/kg and from 0.03 to 2.12 μg/kg, respectively. The LC-MS/MS method has also been successfully applied to 60 maize samples, which were collected from Shaanxi Province of China. Twenty-four of the total 60 samples (40%) were contaminated with at least 1 of these 9 mycotoxins. Occurrence of mycotoxins were 6.7%, 1.7%, 3.3%, 6.7%, 1.7%, 23.3%, and 3.3% for AFB1, AFB2, OTA, ZEA, DON, FB1, and T2-toxin, respectively. The results demonstrated that the procedure was suitable for the simultaneous determination of these mycotoxins in maize matrix.
A sensitive LC-MS/MS method was developed for the simultaneous determination of aflatoxins (AFB1, AFB2, AFG1, AFG2), OTA, DON, ZEA, FB1, and T2-toxin in maize; OASIS HLB cartridge was adopted for cleanup of mycotoxins. This method will have potential analytical applications for other cereals and can be performed for routine analysis in laboratories.