Preparation and Characterization of Novel Lipid Carriers Containing Microalgae Oil for Food Applications

Authors

  • Jia-Li Wang,

    1. Inst. of Oil Crops Research, Chinese Academy of Agricultural Sciences, The Key Lab for Biological Sciences of Oil Crops, Ministry of Agriculture – Hubei Key Laboratory of Lipid Chemistry and Nutrition, Wuhan, Hubei 430062, P. R., China
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  • Xu-Yan Dong,

    Corresponding author
    1. Inst. of Oil Crops Research, Chinese Academy of Agricultural Sciences, The Key Lab for Biological Sciences of Oil Crops, Ministry of Agriculture – Hubei Key Laboratory of Lipid Chemistry and Nutrition, Wuhan, Hubei 430062, P. R., China
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  • Fang Wei,

    1. Inst. of Oil Crops Research, Chinese Academy of Agricultural Sciences, The Key Lab for Biological Sciences of Oil Crops, Ministry of Agriculture – Hubei Key Laboratory of Lipid Chemistry and Nutrition, Wuhan, Hubei 430062, P. R., China
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  • Juan Zhong,

    1. Inst. of Oil Crops Research, Chinese Academy of Agricultural Sciences, The Key Lab for Biological Sciences of Oil Crops, Ministry of Agriculture – Hubei Key Laboratory of Lipid Chemistry and Nutrition, Wuhan, Hubei 430062, P. R., China
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  • Bo Liu,

    1. Wuhan Natl. Laboratory for Optoelectronics – Hubei Bioinformatics and Molecular Imaging Key Laboratory, College of Life Science and Technology, Huazhong Univ. of Science and Technology, Wuhan, Hubei 430074, P. R., China
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  • Ming-Hao Yao,

    1. Wuhan Natl. Laboratory for Optoelectronics – Hubei Bioinformatics and Molecular Imaging Key Laboratory, College of Life Science and Technology, Huazhong Univ. of Science and Technology, Wuhan, Hubei 430074, P. R., China
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  • Mei Yang,

    1. Inst. of Oil Crops Research, Chinese Academy of Agricultural Sciences, The Key Lab for Biological Sciences of Oil Crops, Ministry of Agriculture – Hubei Key Laboratory of Lipid Chemistry and Nutrition, Wuhan, Hubei 430062, P. R., China
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  • Chang Zheng,

    1. Inst. of Oil Crops Research, Chinese Academy of Agricultural Sciences, The Key Lab for Biological Sciences of Oil Crops, Ministry of Agriculture – Hubei Key Laboratory of Lipid Chemistry and Nutrition, Wuhan, Hubei 430062, P. R., China
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  • Siew-Young Quek,

    1. School of Chemical Sciences, The Univ. of Auckland, Auckland 1142, New Zealand
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  • Hong Chen

    Corresponding author
    1. Inst. of Oil Crops Research, Chinese Academy of Agricultural Sciences, The Key Lab for Biological Sciences of Oil Crops, Ministry of Agriculture – Hubei Key Laboratory of Lipid Chemistry and Nutrition, Wuhan, Hubei 430062, P. R., China
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Abstract

This work investigated the suitability of lipid carriers as potential encapsulation method to improve the physical and chemical stability of microalgae oil high in docosahexaenoic acid (DHA). Lipid carriers with various oil contents were successfully prepared by a microfluidization method using stearic acid as solid lipid, microalgae oil as liquid lipid, and poloxamer 188 as surfactant. Results show that the mean particle diameter of the lipid carriers was in the range of 300 to 350 nm with the polydispersity index below 0.2. The lipid carriers were found to have spherical shape when examined under the transmission electron microscope. Data from the encapsulation efficiency and loading capacity indicate high distribution of microalgae oil throughout the lipid carriers and good physical stability as reflected by the particle size and size distribution during storage. Furthermore, the lower DPPH scavenging activity of lipid carriers compared with that of free microalgae oil suggests better chemical stability of microalgae oil encapsulated in lipid carriers. The addition of microalgae oil into lipid phase could disturb the crystalline order and form lattice defects to enable encapsulation of DHA as revealed by the results from differential scanning calorimetery. Current results suggest that this type of novel lipid carriers could be an efficient and promising carrier system for delivery of microalgae oil.

Practical Application

There has been limited report on the application of lipid carriers for encapsulation of microalgae oil with high DHA content for food application. In this study, lipid carriers have been shown to improve the physical and chemical properties of microalgae oil. These lipid carriers could be produced as functional ingredients to be incorporated into functional food products such as frozen desserts, drinks and beverages, and bakery products. High-pressure homogenization, an established method in pharmaceutical industry, could be applied to achieve high-volume production of lipid carriers for food application. The results from this study would, therefore, provide useful insight into the application of DHA-rich microalgae oil in food industry.

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