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Figure S1. The raw spectra of the control water:methanol solution at 50 μg/mL (dark blue line) vs the raw SERS spectra of acetamiprid at 0, 3, 10, and 50 μg/mL in apple juice (average of all data points). The second derivative SERS spectra is shown on the manuscript in Figure 3a; The second derivative spectra was used in the manuscript to help reduce noise and remove baseline, making it easier to see the differences between spectra.

Figure S2. The PLS plot with the root mean square error coefficient of variation (RMSECV) of SERS spectra of different concentrations of acetamiprid in water-methanol (1:1) solvent where RMSECV = 1.14 μg/mL. Data points of 0, 1, 3, 5, 8, 10 μg/mL were used.

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